GPNMB CRISPR/Cas9 KO Plasmid (m): sc-430005
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPNMB CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPNMB genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPNMB CRISPR/Cas9 KO Plasmid (m) | sc-430005 | 20 µg | $397.00 |
Overview
Gpnmb encodes glycoprotein non-metastatic melanoma protein B (GPNMB), a type I transmembrane glycoprotein enriched in macrophages, dendritic cells, osteoclasts, melanocytes, and microglia. GPNMB is implicated in lysosomal and endosomal trafficking, phagocytosis, extracellular matrix remodeling, and cell adhesion programs that shape immune activation and tissue repair. Its expression is often induced by inflammatory cues and cellular stress, linking it to pathways controlling myeloid polarization, bone resorption, and pigmentation biology. Dysregulated GPNMB has been associated with neuroinflammation and neurodegeneration models, metabolic and fibrotic remodeling, and tumor–immune microenvironment phenotypes, making it a useful node for mechanistic studies in mouse systems.
GPNMB CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gpnmb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gpnmb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gpnmb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPNMB protein expression.
This CRISPR knockout system enables efficient generation of Gpnmb-deficient cell models for investigation of GPNMB signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gpnmb exon(s) critical for GPNMB function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gpnmb genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPNMB CRISPR/Cas9 KO Plasmid (m) and GPNMB CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gpnmb locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPNMB HDR Plasmid (m) and GPNMB HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gpnmb homology arms to support homology-directed repair at defined Gpnmb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.