Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GPI CRISPR/Cas9 KO Plasmid (h): sc-402796
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPI CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPI genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPI Antibody (H-10): sc-365066
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPI CRISPR/Cas9 KO Plasmid (h) | sc-402796 | 20 µg | $397.00 |
Overview
Glucose-6-phosphate isomerase (GPI) is a multifunctional enzyme that catalyzes the reversible interconversion of glucose-6-phosphate and fructose-6-phosphate, a central step linking glycolysis and gluconeogenesis. By controlling carbon flux through these pathways, GPI influences cellular ATP production, biosynthetic precursor availability, and redox balance, thereby shaping metabolic adaptation under stress. Beyond its cytosolic metabolic role, GPI has been reported to participate in extracellular signaling contexts, connecting metabolism with cell motility and microenvironmental interactions. Altered GPI activity or expression is associated with metabolic dysregulation and has been studied in contexts including anemia due to inherited enzyme deficiency and metabolic remodeling observed in proliferative disease models.
GPI CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPI gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPI together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPI open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPI protein expression.
This CRISPR knockout system enables efficient generation of GPI-deficient cell models for investigation of GPI signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPI exon(s) critical for GPI function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPI genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPI CRISPR/Cas9 KO Plasmid (h) and GPI CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPI locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPI HDR Plasmid (h) and GPI HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPI homology arms to support homology-directed repair at defined GPI target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.