GPD2 Antibody (5-RY26): sc-134349
- GPD2 Antibody (5-RY26) is a mouse monoclonal IgG1 κ provided at 100 µg/ml
- raised against recombinant GPD2 protein of human origin
- recommended for detection of GPD2 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GPD2 Antibody (5-RY26). This work is in progress.
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GPD2 Antibody (5-RY26) is a mouse monoclonal IgG1 antibody that detects GPD2 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). GPD2, also known as glycerol-3-phosphate dehydrogenase 2 or GDH2, is a 727 amino acid protein that plays a crucial role in cellular metabolism by facilitating the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate while reducing enzyme-bound flavin adenine dinucleotide (FAD). This enzymatic activity is vital for maintaining the balance of NADH and NAD+ in the cell, particularly during glycolysis, and is essential for the proper functioning of the glycerol phosphate shuttle, which is critical for energy production. GPD2 is predominantly localized to the outer surface of the inner mitochondrial membrane, where GPD2 collaborates with GPD1, a cytosolic NAD-linked glycerol-3-phosphate dehydrogenase, to ensure efficient energy metabolism. Notably, GPD2 is highly expressed in human pancreatic islets, where GPD2 is integral to the glucose-sensing mechanism of pancreatic beta cells; a deficiency in GPD2 can lead to impaired insulin release in conditions such as noninsulin-dependent diabetes mellitus. GPD2 exists in two alternatively spliced isoforms and features two EF-hand domains, mapping to human chromosome 2q24.1, highlighting GPD2′s structural complexity and functional significance in metabolic regulation.
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GPD2 Antibody (5-RY26) References:
- Role of glycerol-3-phosphate dehydrogenase 2 in mouse sperm capacitation. | Kota, V., et al. 2010. Mol Reprod Dev. 77: 773-83. PMID: 20602492
- Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses. | Langston, PK., et al. 2019. Nat Immunol. 20: 1186-1195. PMID: 31384058
- Contribution of GPD2/mGPDH to an alternative respiratory chain of the mitochondrial energy metabolism and the stemness in CD133-positive HuH-7 cells. | Mikeli, M., et al. 2020. Genes Cells. 25: 139-148. PMID: 31887237
- Taraxasterol suppresses cell proliferation and boosts cell apoptosis via inhibiting GPD2-mediated glycolysis in gastric cancer. | Zhao, Y., et al. 2021. Cytotechnology. 73: 815-825. PMID: 34776631
- LPL/AQP7/GPD2 promotes glycerol metabolism under hypoxia and prevents cardiac dysfunction during ischemia. | Ishihama, S., et al. 2021. FASEB J. 35: e22048. PMID: 34807469
- A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria. | Wu, S., et al. 2022. Proc Natl Acad Sci U S A. 119: e2121987119. PMID: 35749365
- Mitochondrial GCN5L1 regulates cytosolic redox state and hepatic gluconeogenesis via glycerol phosphate shuttle GPD2. | Meng, J., et al. 2022. Biochem Biophys Res Commun. 621: 1-7. PMID: 35802941
- GPD2: The relationship with cancer and neural stemness. | Mikeli, M., et al. 2023. Cells Dev. 173: 203824. PMID: 36592694
- Non-bioenergetic roles of mitochondrial GPD2 promote tumor progression. | Oh, S., et al. 2023. Theranostics. 13: 438-457. PMID: 36632231
- Histone Lactylation-Driven GPD2 Mediates M2 Macrophage Polarization to Promote Malignant Transformation of Cervical Cancer Progression. | Huang, C., et al. 2024. DNA Cell Biol. 43: 605-618. PMID: 39504115
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPD2 Antibody (5-RY26) | sc-134349 | 100 µg/ml | $333.00 |