GP78-1 Antibody (F-3): sc-166358

GP78-1 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects GP78-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GP78-1 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GP78 serves as a receptor for autocrine motility factor (AMF), a tumor-secreted cytokine that plays a critical role in promoting tumor cell invasion and metastasis. AMF, also known as neuroleukin, enhances tumor cell motility in vitro through interaction with GP78, which is crucial for understanding cancer progression. Notably, GP78 is distributed uniformly across membranes of normal cells but localizes to leading and trailing edges of carcinoma cells, indicating involvement in cellular dynamics during tumorigenesis. In gastric cancer, GP78 expression on cell surface correlates with pathological stage and grade of tumor invasion, highlighting potential as a biomarker for cancer progression. Furthermore, GP78 and AMF interactions may contribute to synaptic mechanisms related to learning and memory, as evidenced by increased expression of both proteins in hippocampi of rats following maze learning. GP78 functions as a RING finger-dependent ubiquitin protein ligase (E3) within endoplasmic reticulum, recruiting UBC7, an ubiquitin-conjugating enzyme (E2), suggesting a link between metastasis and ubiquitin-mediated protein degradation. In humans, alternative splicing of GP78 mRNA results in two distinct isoforms, type I membrane protein and integral membrane protein, further emphasizing complexity and significance in cellular processes.

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GP78-1 Antibody (F-3) References:

1. The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum.  |  Fang, S., et al. 2001. Proc Natl Acad Sci U S A. 98: 14422-7. PMID: 11724934
2. A link between maze learning and hippocampal expression of neuroleukin and its receptor gp78.  |  Luo, Y., et al. 2002. J Neurochem. 80: 354-61. PMID: 11902125
3. Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor.  |  Watanabe, H., et al. 1991. J Biol Chem. 266: 13442-8. PMID: 1649192
4. CHIP and gp78-mediated ubiquitination of CYP3A4: Implications for the pharmacology of anticancer agents.  |  Peer, CJ., et al. 2011. Cancer Biol Ther. 11: 549-51. PMID: 21270532
5. Autocrine motility factor-receptor in human bladder-carcinoma - gene-expression, loss of cell-contact regulation and chromosomal mapping.  |  Silletti, S., et al. 1993. Int J Oncol. 3: 801-7. PMID: 21573434
6. Regulation of mitophagy by the Gp78 E3 ubiquitin ligase.  |  Fu, M., et al. 2013. Mol Biol Cell. 24: 1153-62. PMID: 23427266
7. SVIP regulates Z variant alpha-1 antitrypsin retro-translocation by inhibiting ubiquitin ligase gp78.  |  Khodayari, N., et al. 2017. PLoS One. 12: e0172983. PMID: 28301499
8. GP78 Cooperates with Dual-Specificity Phosphatase 1 To Stimulate Epidermal Growth Factor Receptor-Mediated Extracellular Signal-Regulated Kinase Signaling.  |  Kho, DH., et al. 2019. Mol Cell Biol. 39: PMID: 31061093
9. Ubiquitination of NLRP3 by gp78/Insig-1 restrains NLRP3 inflammasome activation.  |  Xu, T., et al. 2022. Cell Death Differ. 29: 1582-1595. PMID: 35110683
10. Basal Gp78-dependent mitophagy promotes mitochondrial health and limits mitochondrial ROS.  |  Alan, P., et al. 2022. Cell Mol Life Sci. 79: 565. PMID: 36284011
11. Gp78 deficiency in hepatocytes alleviates hepatic ischemia-reperfusion injury via suppressing ACSL4-mediated ferroptosis.  |  Li, C., et al. 2023. Cell Death Dis. 14: 810. PMID: 38065978
12. Expression of autocrine motility factor receptor correlates with disease progression in human gastric cancer.  |  Hirono, Y., et al. 1996. Br J Cancer. 74: 2003-7. PMID: 8980404

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GP78-1 Antibody (F-3) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-GP78-1 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GP78-1 Antibody (F-3) Specifications

Alternate Product Name: GP78-1

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 87-116

Epitope Target: GP78-1

Epitope Species: human

Product Reactivity/Detection: GP78 isoform 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for GP78-1 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin