GP-39 Antibody (E-11) recommended for detection of GP-39 of mouse origin by WB, IP, IF, IHC(P) and ELISA

GP-39 Antibody (E-11): sc-376910

GP-39 Antibody (E-11) is rated 4.3 out of 5 by 3.
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Datasheets

    • GP-39 Antibody (E-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 136-178 mapping within an internal region of GP-39 of mouse origin
    • GP-39 Antibody (E-11) is recommended for detection of GP-39 of mouse origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-GP-39 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GP-39 (E-11): sc-376910.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for GP-39 Antibody (E-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with GP-39 Antibody (E-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I am using GP-39 (E-11): sc-376910 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Not working yet We haven´t been able to obtain specific staining in IHC
Date published: 2018-05-08
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by from Produced positive Western Blot data of GP Produced positive Western Blot data of GP-39 expression in non-transfected and mouse GP-39 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-12-03
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