Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
golgin 97 Double Nickase Plasmid (h): sc-403152-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- golgin 97 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- golgin 97 Double Nickase Plasmid (h) and golgin 97 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GOLGA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: golgin 97 Antibody (CDFX): sc-59820
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
golgin 97 Double Nickase Plasmid (h) | sc-403152-NIC | 20 µg | $410.00 | |||
golgin 97 Double Nickase Plasmid (h2) | sc-403152-NIC-2 | 20 µg | $410.00 |
GOLGA1 encodes golgin 97, a coiled-coil golgin that localizes to the trans-Golgi network and functions as a vesicle tether to coordinate endosome-to-Golgi retrograde trafficking and Golgi membrane organization. Through interactions with small GTPases and tethering factors, golgin 97 helps maintain sorting fidelity, cargo recycling, and polarized trafficking that support secretion and membrane protein homeostasis. Disruption of Golgi tethering and retrograde transport is broadly linked to cellular stress responses, altered receptor signaling, and defects in protein processing. As a component of the endomembrane trafficking network, golgin 97 is frequently investigated in studies of Golgi integrity, organelle dynamics, and trafficking-dependent phenotypes relevant to neurodegeneration, infection biology, and cancer cell biology.
golgin 97 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GOLGA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GOLGA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GOLGA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GOLGA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.