golgin 84 Antibody (D-5): sc-365337
- golgin 84 Antibody (D-5) is a mouse monoclonal IgG1 κ golgin 84 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 343-625 mapping within an internal region of golgin 84 of human origin
- golgin 84 Antibody (D-5) is recommended for detection of golgin 84 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-golgin 84 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for golgin 84 Antibody (D-5). This work is in progress.
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golgin 84 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects golgin 84 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-golgin 84 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Golgin 84, also known as GOLGA5 (Golgi autoantigen, subfamily A, member 5), RFG5 (RET-fused gene 5 protein), or GOLIM5, is a crucial single-pass type II coiled-coil membrane protein that is ubiquitously expressed and primarily localized to the Golgi apparatus, particularly on membranes at the cis side of the Golgi stack. This localization is vital for maintaining Golgi structure and facilitating the formation of Golgi stacks and ribbons, which are essential for proper protein processing and trafficking. The depletion of golgin 84 results in fragmentation of the Golgi ribbon and a significant reduction in the efficiency of protein transport, highlighting its critical role in cellular function. Furthermore, golgin 84 interacts with active Rab 1 and associates with CASP (an isoform of CDP) within a golgin-tethering complex, which is believed to be involved in intra-Golgi retrograde transport. Notably, during mitosis, golgin 84 undergoes extensive phosphorylation, and chromosomal translocations involving the gene encoding golgin 84 have been linked to various cancer tissues, producing chimeric proteins such as Ret-II and PTC5 that may contribute to tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
golgin 84 Antibody (D-5) References:
- The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation. | Diao, A., et al. 2003. J Cell Biol. 160: 201-12. PMID: 12538640
- Golgin tethers define subpopulations of COPI vesicles. | Malsam, J., et al. 2005. Science. 307: 1095-8. PMID: 15718469
- Tethering assays for COPI vesicles mediated by golgins. | Satoh, A., et al. 2005. Methods Enzymol. 404: 125-34. PMID: 16413264
- Preparation and characterization of recombinant golgin tethers. | Satoh, A., et al. 2005. Methods Enzymol. 404: 279-96. PMID: 16413277
- Localization and domain characterization of Arabidopsis golgin candidates. | Latijnhouwers, M., et al. 2007. J Exp Bot. 58: 4373-86. PMID: 18182439
- Activation of the ret-II oncogene without a sequence encoding a transmembrane domain and transforming activity of two ret-II oncogene products differing in carboxy-termini due to alternative splicing. | Ishizaka, Y., et al. 1989. Oncogene. 4: 789-94. PMID: 2734021
- Detection of a novel type of RET rearrangement (PTC5) in thyroid carcinomas after Chernobyl and analysis of the involved RET-fused gene RFG5. | Klugbauer, S., et al. 1998. Cancer Res. 58: 198-203. PMID: 9443391
- Identification and characterization of golgin-84, a novel Golgi integral membrane protein with a cytoplasmic coiled-coil domain. | Bascom, RA., et al. 1999. J Biol Chem. 274: 2953-62. PMID: 9915833
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
golgin 84 Antibody (D-5) | sc-365337 | 200 µg/ml | $316.00 | |||
golgin 84 Antibody (D-5) AC | sc-365337 AC | 500 µg/ml, 25% agarose | $416.00 | |||
golgin 84 Antibody (D-5) HRP | sc-365337 HRP | 200 µg/ml | $316.00 | |||
golgin 84 Antibody (D-5) FITC | sc-365337 FITC | 200 µg/ml | $330.00 | |||
golgin 84 Antibody (D-5) PE | sc-365337 PE | 200 µg/ml | $343.00 | |||
golgin 84 Antibody (D-5) Alexa Fluor® 488 | sc-365337 AF488 | 200 µg/ml | $357.00 | |||
golgin 84 Antibody (D-5) Alexa Fluor® 546 | sc-365337 AF546 | 200 µg/ml | $357.00 | |||
golgin 84 Antibody (D-5) Alexa Fluor® 594 | sc-365337 AF594 | 200 µg/ml | $357.00 | |||
golgin 84 Antibody (D-5) Alexa Fluor® 647 | sc-365337 AF647 | 200 µg/ml | $357.00 | |||
golgin 84 Antibody (D-5) Alexa Fluor® 680 | sc-365337 AF680 | 200 µg/ml | $357.00 | |||
golgin 84 Antibody (D-5) Alexa Fluor® 790 | sc-365337 AF790 | 200 µg/ml | $357.00 |