golgin 245 Double Nickase Plasmid (h): sc-404412-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- golgin 245 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- golgin 245 Double Nickase Plasmid (h) and golgin 245 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GOLGA4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: golgin 245 Antibody (G-7): sc-514775
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
golgin 245 Double Nickase Plasmid (h) | sc-404412-NIC | 20 µg | $410.00 | |||
golgin 245 Double Nickase Plasmid (h2) | sc-404412-NIC-2 | 20 µg | $410.00 |
GOLGA4 encodes golgin 245, a coiled-coil Golgi matrix protein that contributes to Golgi ribbon organization and vesicle tethering required for efficient cargo sorting through the secretory pathway. Golgin 245 participates in membrane trafficking processes that coordinate retrograde and anterograde transport between the Golgi and endosomal compartments, supporting proper distribution of glycosylation enzymes and maintenance of Golgi architecture. Disruption of Golgi tethering and trafficking networks is associated with altered cell polarity, stress signaling, and defects in protein processing that are frequently observed in cancer and neurodegenerative disease contexts. As a marker and regulator of Golgi dynamics, golgin 245 is widely used to interrogate organelle organization, vesicle transport, and proteostasis-related pathways in human cells.
golgin 245 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GOLGA4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GOLGA4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GOLGA4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GOLGA4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.