Golgi Marker Antibody (AE-6): sc-58770

Golgi Marker Antibody (AE-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Golgi Marker protein of human origin by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Golgi Marker (AE-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Golgi Marker protein plays a crucial role in cellular processing and trafficking of proteins and lipids, making Golgi Marker protein essential for maintaining cellular homeostasis and function. Located within the Golgi apparatus, Golgi Marker protein is involved in modification, sorting, and packaging of macromolecules synthesized in the endoplasmic reticulum before dispatching to final destinations, either inside or outside the cell. Golgi apparatus is structured as a series of flattened, membrane-bound sacs known as cisternae, organized into three functional regions: cis face, medial region, and trans face. Each region contains specific enzymes that facilitate post-translational modifications necessary for protein maturation, such as glycosylation and phosphorylation, which are vital for stability and activity. Golgi apparatus is particularly prominent in secretory cells, such as plasma B cells, where Golgi Marker protein is responsible for antibody production. Understanding Golgi apparatus dynamics and markers is critical for researchers studying cellular processes, disease mechanisms, and therapeutic targets.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Golgi Marker Antibody (AE-6) References:

1. Re-defining the Golgi complex in Plasmodium falciparum using the novel Golgi marker PfGRASP.  |  Struck, NS., et al. 2005. J Cell Sci. 118: 5603-13. PMID: 16306223
2. H+-translocating ATPase in Golgi apparatus. Characterization as vacuolar H+-ATPase and its subunit structures.  |  Moriyama, Y. and Nelson, N. 1989. J Biol Chem. 264: 18445-50. PMID: 2530222
3. Dynamic distribution of the Golgi marker thiamine pyrophosphatase is modulated by brefeldin A in rat hepatoma cells.  |  Fujiwara, T., et al. 1989. Cell Struct Funct. 14: 605-16. PMID: 2559814
4. Characterization of protein transport between successive compartments of the Golgi apparatus: asymmetric properties of donor and acceptor activities in a cell-free system.  |  Balch, WE. and Rothman, JE. 1985. Arch Biochem Biophys. 240: 413-25. PMID: 2990347
5. Dictyosome-like structures from guinea-pig testes lack galactosyltransferase, a Golgi apparatus marker.  |  Morré, DJ., et al. 1985. Cell Tissue Res. 240: 35-40. PMID: 3922620
6. Isolation and characterization of endoplasmic reticulum and Golgi apparatus from hepatocyte nodules in male wistar rats.  |  Eriksson, LC., et al. 1983. Cancer Res. 43: 3335-47. PMID: 6189597
7. Transport of sugar nucleotides into rat liver Golgi. A new Golgi marker activity.  |  Sommers, LW. and Hirschberg, CB. 1982. J Biol Chem. 257: 10811-7. PMID: 7050120
8. Plasmodium falciparum exports the Golgi marker sphingomyelin synthase into a tubovesicular network in the cytoplasm of mature erythrocytes.  |  Elmendorf, HG. and Haldar, K. 1994. J Cell Biol. 124: 449-62. PMID: 8106545
9. Characterization of KIF1C, a new kinesin-like protein involved in vesicle transport from the Golgi apparatus to the endoplasmic reticulum.  |  Dorner, C., et al. 1998. J Biol Chem. 273: 20267-75. PMID: 9685376

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Golgi Marker Antibody (AE-6) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Golgi Marker Antibody (AE-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Golgi Marker Antibody (AE-6) Specifications

Alternate Product Name: Golgi Marker

Product Clone Name: AE-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target:

Epitope Species: human

Product Reactivity/Detection: Golgi Zone

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Golgi Marker Antibody (AE-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin