GMEB-1 Antibody (H-2): sc-376775
- GMEB-1 Antibody (H-2) is a mouse monoclonal IgG1 κ GMEB-1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 225-319 mapping within an internal region of GMEB-1 of mouse origin
- GMEB-1 Antibody (H-2) is recommended for detection of GMEB-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
- Anti-GMEB-1 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-376775 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GMEB-1 Antibody (H-2) for WB and IHC(P) applications. These reagents are now offered in bundles with GMEB-1 Antibody (H-2) (see ordering information below).
GMEB-1 Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects GMEB-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GMEB-1 (H-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GMEB-1, or Glucocorticoid modulatory element-binding protein 1, is a 573 amino acid protein characterized by one SAND domain and belongs to the KDWK family of combinatorial transcription modulators. GMEB-1 plays a crucial role in regulating gene expression in response to glucocorticoids by binding to glucocorticoid modulatory elements in target gene promoters, enhancing transcriptional activity. GMEB-1 is found in both cytoplasm and nucleus, where GMEB-1 forms a heterodimer with GMEB-2, facilitating parvovirus DNA replication. GMEB-1 also interacts with initiator procaspases to inhibit caspase-induced apoptosis, highlighting GMEB-1′s importance in cellular survival mechanisms. Due to alternative splicing, GMEB-1 exists in two isoforms, adding complexity to GMEB-1′s functional roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GMEB-1 Antibody (H-2) References:
- Cloning and characterization of hGMEB1, a novel glucocorticoid modulatory element binding protein. | Thériault, JR., et al. 1999. FEBS Lett. 452: 170-6. PMID: 10386584
- Two new members of the emerging KDWK family of combinatorial transcription modulators bind as a heterodimer to flexibly spaced PuCGPy half-sites. | Christensen, J., et al. 1999. Mol Cell Biol. 19: 7741-50. PMID: 10523663
- Properties of the glucocorticoid modulatory element binding proteins GMEB-1 and -2: potential new modifiers of glucocorticoid receptor transactivation and members of the family of KDWK proteins. | Kaul, S., et al. 2000. Mol Endocrinol. 14: 1010-27. PMID: 10894151
- A consensus DNA recognition motif for two KDWK transcription factors identifies flexible-length, CpG-methylation sensitive cognate binding sites in the majority of human promoters. | Burnett, E., et al. 2001. J Mol Biol. 314: 1029-39. PMID: 11743720
- Crystal structure and nuclear magnetic resonance analyses of the SAND domain from glucocorticoid modulatory element binding protein-1 reveals deoxyribonucleic acid and zinc binding regions. | Surdo, PL., et al. 2003. Mol Endocrinol. 17: 1283-95. PMID: 12702733
- Regulation of procaspase-2 by glucocorticoid modulatory element-binding protein 1 through the interaction with caspase recruitment domain. | Tsuruma, K., et al. 2004. Biochem Biophys Res Commun. 325: 1246-51. PMID: 15555560
- Glucocorticoid modulatory element-binding protein 1 binds to initiator procaspases and inhibits ischemia-induced apoptosis and neuronal injury. | Tsuruma, K., et al. 2006. J Biol Chem. 281: 11397-404. PMID: 16497673
- The factor binding to the glucocorticoid modulatory element of the tyrosine aminotransferase gene is a novel and ubiquitous heteromeric complex. | Oshima, H., et al. 1995. J Biol Chem. 270: 21893-901. PMID: 7665613
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GMEB-1 Antibody (H-2) | sc-376775 | 200 µg/ml | $322.00 | |||
GMEB-1 Antibody (H-2): m-IgG Fc BP-HRP Bundle | sc-527548 | 200 µg Ab; 10 µg BP | $361.00 | |||
GMEB-1 Antibody (H-2): m-IgG1 BP-HRP Bundle | sc-532921 | 200 µg Ab; 20 µg BP | $361.00 | |||
GMEB-1 Antibody (H-2) X | sc-376775 X | 200 µg/0.1 ml | $322.00 | |||
GMEB-1 Antibody (H-2) AC | sc-376775 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GMEB-1 Antibody (H-2) HRP | sc-376775 HRP | 200 µg/ml | $322.00 | |||
GMEB-1 Antibody (H-2) FITC | sc-376775 FITC | 200 µg/ml | $336.00 | |||
GMEB-1 Antibody (H-2) PE | sc-376775 PE | 200 µg/ml | $349.00 | |||
GMEB-1 Antibody (H-2) Alexa Fluor® 488 | sc-376775 AF488 | 200 µg/ml | $364.00 | |||
GMEB-1 Antibody (H-2) Alexa Fluor® 546 | sc-376775 AF546 | 200 µg/ml | $364.00 | |||
GMEB-1 Antibody (H-2) Alexa Fluor® 594 | sc-376775 AF594 | 200 µg/ml | $364.00 | |||
GMEB-1 Antibody (H-2) Alexa Fluor® 647 | sc-376775 AF647 | 200 µg/ml | $364.00 | |||
GMEB-1 Antibody (H-2) Alexa Fluor® 680 | sc-376775 AF680 | 200 µg/ml | $364.00 | |||
GMEB-1 Antibody (H-2) Alexa Fluor® 790 | sc-376775 AF790 | 200 µg/ml | $364.00 |