glypican-4 Antibody (AT51E3): sc-517403

glypican-4 Antibody (AT51E3) is a mouse monoclonal IgG2a kappa light chain antibody that detects the glypican-4 protein of human origin by western blotting (WB), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). anti-glypican-4 antibody (AT51E3) is available as a non-conjugated format. Glypican-4, also known as K-glypican, is a 556 amino acid cell surface proteoglycan that plays a crucial role in the development of the central nervous system and the formation of kidney tubules. Glypican-4 is anchored to the cell membrane via a glycosylphosphatidylinositol moiety and is involved in regulating cell growth and division through interaction with various signaling molecules. Notably, glypican-4 is implicated in the modulation of fibroblast growth factor 2 (FGF-2) activity during cortical neurogenesis, highlighting its importance in neural development. Following proteolytic cleavage, glypican-4 is released into the extracellular space, where glypican-4 can influence cellular signaling pathways. The gene encoding glypican-4 is located on human chromosome Xq26.2 and mouse chromosome X A5, and glypican-4 deletion has been associated with Simpson-Golabi-Behmel syndrome, an X-linked condition that shares clinical features with Beckwith-Wiedemann syndrome. This makes glypican-4 not only a significant marker for research but also a potential target for understanding developmental disorders.

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glypican-4 Antibody (AT51E3) References:

1. Expression of glypican-4 in haematopoietic-progenitor and bone-marrow-stromal cells.  |  Siebertz, B., et al. 1999. Biochem J. 344 Pt 3: 937-43. PMID: 10585884
2. Mutational analysis of the GPC3/GPC4 glypican gene cluster on Xq26 in patients with Simpson-Golabi-Behmel syndrome: identification of loss-of-function mutations in the GPC3 gene.  |  Veugelers, M., et al. 2000. Hum Mol Genet. 9: 1321-8. PMID: 10814714
3. Glypican-4 is an FGF2-binding heparan sulfate proteoglycan expressed in neural precursor cells.  |  Hagihara, K., et al. 2000. Dev Dyn. 219: 353-67. PMID: 11066092
4. Cell surface glypicans are low-affinity endostatin receptors.  |  Karumanchi, SA., et al. 2001. Mol Cell. 7: 811-22. PMID: 11336704
5. K-glypican: a novel GPI-anchored heparan sulfate proteoglycan that is highly expressed in developing brain and kidney.  |  Watanabe, K., et al. 1995. J Cell Biol. 130: 1207-18. PMID: 7657705
6. GPC4, the gene for human K-glypican, flanks GPC3 on xq26: deletion of the GPC3-GPC4 gene cluster in one family with Simpson-Golabi-Behmel syndrome.  |  Veugelers, M., et al. 1998. Genomics. 53: 1-11. PMID: 9787072
7. Glypican 3 and glypican 4 are juxtaposed in Xq26.1.  |  Huber, R., et al. 1998. Gene. 225: 9-16. PMID: 9931407