glypican-3 Antibody (F-3): sc-390587

Glypican-3 Antibody (F-3) is a mouse monoclonal IgM antibody that detects glypican-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-glypican-3 antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glypican-3 (GPC3) is an integral membrane protein that plays a crucial role in regulating cell growth and development, particularly during embryogenesis. GPC3 is a member of the glypican family of heparan sulfate proteoglycans, which are attached to the cell membrane via a glycosyl-phosphatidyl-inositol (GPI) anchor. GPC3 is primarily expressed in embryonic mesodermal tissues such as the lung, liver, and kidney, where GPC3 interacts with growth factors like insulin-like growth factor II (IGF-II) and fibroblast growth factor 2 (FGF-2) to modulate cellular signaling pathways that influence tissue and organ growth. Mutations in the GPC3 gene are associated with Simpson-Golabi-Behmel syndrome (SGBS), a recessive X-linked condition characterized by pre- and post-natal overgrowth, highlighting GPC3′s significance in developmental processes. GPC3 expression can be downregulated through mechanisms such as promoter hypermethylation or the repression of specific transcription factors, which may have implications in various pathologies, including cancer.

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glypican-3 Antibody (F-3) References:

1. Frequent silencing of the GPC3 gene in ovarian cancer cell lines.  |  Lin, H., et al. 1999. Cancer Res. 59: 807-10. PMID: 10029067
2. Glypican-3-deficient mice exhibit developmental overgrowth and some of the abnormalities typical of Simpson-Golabi-Behmel syndrome.  |  Cano-Gauci, DF., et al. 1999. J Cell Biol. 146: 255-64. PMID: 10402475
3. Expression of GPC3, an X-linked recessive overgrowth gene, is silenced in malignant mesothelioma.  |  Murthy, SS., et al. 2000. Oncogene. 19: 410-6. PMID: 10656689
4. Increased Glypican-3 immunostaining is associated with longer survival outcomes in colorectal carcinoma.  |  Al-Maghrabi, J., et al. 2022. Pol J Pathol. 73: 14-20. PMID: 35848476
5. Frequently expressed glypican-3 as a promising novel therapeutic target for osteosarcomas.  |  Nie, JH., et al. 2022. Cancer Sci. 113: 3618-3632. PMID: 35946078
6. Serum glypican-3 for the prediction of survival in patients with hepatocellular carcinoma.  |  Nicolosi, A., et al. 2022. Minerva Gastroenterol (Torino). 68: 378-386. PMID: 36222678
7. Enhanced glypican-3-targeted identification of hepatocellular carcinoma with liver fibrosis by pre-degrading excess fibrotic collagen.  |  Zhuo, J., et al. 2023. Acta Biomater. 158: 435-448. PMID: 36603729
8. The role and mechanism of HIF-1α-mediated glypican-3 secretion in hypoxia-induced tumor progression in hepatocellular carcinoma.  |  Wang, P., et al. 2024. Cell Signal. 114: 111007. PMID: 38081444
9. Mutations in GPC3, a glypican gene, cause the Simpson-Golabi-Behmel overgrowth syndrome.  |  Pilia, G., et al. 1996. Nat Genet. 12: 241-7. PMID: 8589713
10. OCI-5/rat glypican-3 binds to fibroblast growth factor-2 but not to insulin-like growth factor-2.  |  Song, HH., et al. 1997. J Biol Chem. 272: 7574-7. PMID: 9065409
11. Expression of OCI-5/glypican 3 during intestinal morphogenesis: regulation by cell shape in intestinal epithelial cells.  |  Li, M., et al. 1997. Exp Cell Res. 235: 3-12. PMID: 9281346
12. OCI-5/GPC3, a glypican encoded by a gene that is mutated in the Simpson-Golabi-Behmel overgrowth syndrome, induces apoptosis in a cell line-specific manner.  |  Gonzalez, AD., et al. 1998. J Cell Biol. 141: 1407-14. PMID: 9628896

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-glypican-3 Antibody (F-3) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-glypican-3 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

glypican-3 Antibody (F-3) Specifications

Alternate Product Name: GPC3

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 48-74

Epitope Target: glypican-3

Epitope Species: human

Product Reactivity/Detection: glypican-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for glypican-3 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin