Glyoxalase II Antibody (F-9): sc-166781
- Glyoxalase II Antibody (F-9) is a mouse monoclonal IgG1 κ Glyoxalase II antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 61-110 mapping within an internal region of Glyoxalase II of human origin
- Glyoxalase II Antibody (F-9) is recommended for detection of Glyoxalase II of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Glyoxalase II Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Glyoxalase II Antibody (F-9) for WB applications. These reagents are now offered in bundles with Glyoxalase II Antibody (F-9) (see ordering information below).
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Glyoxalase II Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Glyoxalase II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Glyoxalase II antibody (F-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glyoxalase II (F-9) antibody targets a protein that plays a crucial role in detoxifying harmful glucose degradation products, functioning as a thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to produce reduced glutathione and D-lactic acid. This enzymatic activity is vital for maintaining cellular health, particularly in oxidative stress and metabolic regulation. Glyoxalase II primarily resides in mitochondria and cytosol of mammalian cells, existing as a monomer and binding two zinc ions per subunit, a structural feature essential for enzymatic function. Notably, both Glyoxalase I and Glyoxalase II show increased activity in breast cancer tissues compared to matched unaffected tissues, indicating potential as therapeutic targets and suggesting glyoxalase inhibitor drugs may be effective in cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Glyoxalase II Antibody (F-9) References:
- Crystal structure of human glyoxalase II and its complex with a glutathione thiolester substrate analogue. | Cameron, AD., et al. 1999. Structure. 7: 1067-78. PMID: 10508780
- Expression of glyoxalase I and II in normal and breast cancer tissues. | Rulli, A., et al. 2001. Breast Cancer Res Treat. 66: 67-72. PMID: 11368412
- The Human hydroxyacylglutathione hydrolase (HAGH) gene encodes both cytosolic and mitochondrial forms of glyoxalase II. | Cordell, PA., et al. 2004. J Biol Chem. 279: 28653-61. PMID: 15117945
- Identification of glyoxalase-I as a protein marker in a mouse model of extremes in trait anxiety. | Krömer, SA., et al. 2005. J Neurosci. 25: 4375-84. PMID: 15858064
- Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione. | Yadav, SK., et al. 2005. Biochem Biophys Res Commun. 337: 61-7. PMID: 16176800
- Specificity of the trypanothione-dependent Leishmania major glyoxalase I: structure and biochemical comparison with the human enzyme. | Ariza, A., et al. 2006. Mol Microbiol. 59: 1239-48. PMID: 16430697
- Crystallization and preliminary X-ray analysis of Leishmania major glyoxalase I. | Ariza, A., et al. 2005. Acta Crystallogr Sect F Struct Biol Cryst Commun. 61: 769-72. PMID: 16511153
- Molecular cloning, heterologous expression, and characterization of human glyoxalase II. | Ridderström, M., et al. 1996. J Biol Chem. 271: 319-23. PMID: 8550579
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glyoxalase II Antibody (F-9) | sc-166781 | 200 µg/ml | $322.00 | |||
Glyoxalase II Antibody (F-9): m-IgG Fc BP-HRP Bundle | sc-527188 | 200 µg Ab; 10 µg BP | $361.00 | |||
Glyoxalase II Antibody (F-9): m-IgG1 BP-HRP Bundle | sc-532561 | 200 µg Ab; 20 µg BP | $361.00 | |||
Glyoxalase II Antibody (F-9) AC | sc-166781 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Glyoxalase II Antibody (F-9) HRP | sc-166781 HRP | 200 µg/ml | $322.00 | |||
Glyoxalase II Antibody (F-9) FITC | sc-166781 FITC | 200 µg/ml | $336.00 | |||
Glyoxalase II Antibody (F-9) PE | sc-166781 PE | 200 µg/ml | $349.00 | |||
Glyoxalase II Antibody (F-9) Alexa Fluor® 488 | sc-166781 AF488 | 200 µg/ml | $364.00 | |||
Glyoxalase II Antibody (F-9) Alexa Fluor® 546 | sc-166781 AF546 | 200 µg/ml | $364.00 | |||
Glyoxalase II Antibody (F-9) Alexa Fluor® 594 | sc-166781 AF594 | 200 µg/ml | $364.00 | |||
Glyoxalase II Antibody (F-9) Alexa Fluor® 647 | sc-166781 AF647 | 200 µg/ml | $364.00 | |||
Glyoxalase II Antibody (F-9) Alexa Fluor® 680 | sc-166781 AF680 | 200 µg/ml | $364.00 | |||
Glyoxalase II Antibody (F-9) Alexa Fluor® 790 | sc-166781 AF790 | 200 µg/ml | $364.00 |