Glycophorin A/B Antibody (HIR2): sc-51603

Glycophorin A/B Antibody (HIR2) is a mouse monoclonal IgG2b antibody that detects Glycophorin A/B in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-Glycophorin A/B antibody (HIR2) is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. Glycophorin A and B are integral sialoglycoproteins located in the human erythrocyte membrane, playing a crucial role in defining blood group phenotypes through their antigenic determinants for the MN, Ss, and Gerbich blood groups. The structure of these proteins spans the membrane once, with their amino-terminal ends exposed to the extracellular environment, facilitating interactions with other molecules and cells. The human Glycophorin A gene, located on chromosome 4q31.21, consists of 7 exons and encodes a 150 amino acid protein that is 97% homologous to Glycophorin B, which encodes a 91 amino acid protein. Glycophorin C, another member of this family, is encoded by a gene on chromosome 2q14.3 and can produce two protein isoforms, further contributing to the complexity of blood group antigen expression. Understanding the structure and function of these glycophorins is essential for blood transfusion compatibility and the study of hematological disorders, making Glycophorin A/B monoclonal antibody (HIR2) a valuable tool in both clinical and research settings.

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Glycophorin A/B Antibody (HIR2) References:

1. Immunohistochemical identification of erythroid precursors in paraffin embedded bone marrow sections: spectrin is a superior marker to glycophorin.  |  Sadahira, Y., et al. 1999. J Clin Pathol. 52: 919-21. PMID: 10711257
2. In vivo detection of hetero-association of glycophorin-A and its mutants within the membrane.  |  Gerber, D. and Shai, Y. 2001. J Biol Chem. 276: 31229-32. PMID: 11402026
3. Distinct regions of human glycophorin A enhance human red cell anion exchanger (band 3; AE1) transport function and surface trafficking.  |  Young, MT. and Tanner, MJ. 2003. J Biol Chem. 278: 32954-61. PMID: 12813056
4. Quantification of glycophorin A and glycophorin B on normal human RBCs by flow cytometry.  |  de Isla, NG., et al. 2003. Transfusion. 43: 1145-52. PMID: 12869123
5. Altered structure and anion transport properties of band 3 (AE1, SLC4A1) in human red cells lacking glycophorin A.  |  Bruce, LJ., et al. 2004. J Biol Chem. 279: 2414-20. PMID: 14604989
6. Structural adaptation of the glycophorin A transmembrane homodimer to D-amino acid modifications.  |  Gerber, D., et al. 2004. J Mol Biol. 339: 243-50. PMID: 15123435
7. Complex interactions at the helix-helix interface stabilize the glycophorin A transmembrane dimer.  |  Doura, AK. and Fleming, KG. 2004. J Mol Biol. 343: 1487-97. PMID: 15491626
8. Characterization of alternatively spliced transcript variants of glycophorin A and glycophorin B genes in Chinese blood donors.  |  Liang, Y., et al. 2022. Vox Sang. 117: 715-723. PMID: 35138639
9. Differential enzymatic deglycosylation reveals attachment of red cell B antigen onto the carbohydrate moiety of glycophorin A and glycophorin B.  |  Hsu, K. 2023. Vox Sang. 118: 147-152. PMID: 36510386
10. Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia.  |  Andersson, LC., et al. 1979. Int J Cancer. 24: 717-20. PMID: 397196
11. Glycophorin A expression in malignant hematopoiesis.  |  Liszka, K., et al. 1983. Am J Hematol. 15: 219-26. PMID: 6638008
12. Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers.  |  Nakahata, T. and Okumura, N. 1994. Leuk Lymphoma. 13: 401-9. PMID: 8069185

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Glycophorin A/B Antibody (HIR2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Glycophorin A/B Antibody (HIR2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Glycophorin A/B Antibody (HIR2) Specifications

Alternate Product Name: Glycophorin A/B

Product Clone Name: HIR2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: Glycophorin A

Epitope Species: human

Product Reactivity/Detection: an N-terminal homologous portion of Glycophorin A and Glycophorin B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 100 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Glycophorin A/B Antibody (HIR2): PE: Phycoerythrin

The preferred Glycophorin A antibody is Glycophorin A Antibody (R10): sc-53905.