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Glycophorin A/B Antibody (5F60): sc-71158

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  • Glycophorin A/B Antibody (5F60) is a mouse monoclonal IgG2b Glycophorin A/B antibody provided at 100 µg/ml
  • raised against N-terminal region of Glycophorin A of human origin
  • recommended for detection of an N-terminal homologous portion of Glycophorin A and Glycophorin B of human origin by WB, IP, IF and FCM
  • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • See Glycophorin A (R10): sc-53905 for Glycophorin A/B antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Glycophorin A/B Antibody (5F60). This work is in progress.

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    Glycophorin A/B Antibody (5F60) is a mouse monoclonal IgG2b antibody that detects Glycophorin A/B protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-Glycophorin A/B antibody (5F60) is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE). Glycophorin A and B are integral sialoglycoproteins located in the human erythrocyte membrane, playing a crucial role in defining blood group phenotypes through their antigenic determinants for the MN, Ss, and Gerbich blood groups. These proteins span the membrane once, with their amino-terminal ends exposed to the extracellular environment, which is vital for their interaction with other cells and proteins in the bloodstream. The Glycophorin A gene, located on chromosome 4q31.21, consists of seven exons and encodes a 150 amino acid protein that is 97% homologous to Glycophorin B, which encodes a 91 amino acid protein. The presence of these glycoproteins not only contributes to the structural integrity of red blood cells but also influences their interactions with pathogens and immune cells, making them significant in transfusion medicine and understanding blood group-related diseases. Glycophorin A/B (5F60) monoclonal antibody is an essential tool for researchers studying erythrocyte biology and blood group antigen expression.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Glycophorin A/B Antibody (5F60) References:

    1. Section 3: Epitope determination of monoclonal antibodies to glycophorin A and glycophorin B. Coordinator's report. Antibodies to antigens located on glycophorins and band 3.  |  Reid, ME., et al. 2002. Transfus Clin Biol. 9: 63-72. PMID: 11889901
    2. Contribution of MNS to the study of glycophorin A and glycophorin B.  |  Reid, M. 1999. Immunohematology. 15: 5-9. PMID: 15373530
    3. Studies on human red-cell membrane glycophorin A and glycophorin B genes in glycophorin-deficient individuals.  |  Tate, CG., et al. 1989. Biochem J. 263: 993-6. PMID: 2597142
    4. Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences.  |  Kudo, S. and Fukuda, M. 1989. Proc Natl Acad Sci U S A. 86: 4619-23. PMID: 2734312
    5. The Malaria-Protective Human Glycophorin Structural Variant DUP4 Shows Somatic Mosaicism and Association with Hemoglobin Levels.  |  Algady, W., et al. 2018. Am J Hum Genet. 103: 769-776. PMID: 30388403
    6. Molecular Detection of Glycophorins A and B Variant Phenotypes and their Clinical Relevance.  |  Hassan, SN., et al. 2019. Transfus Med Rev. 33: 118-124. PMID: 30910255
    7. Alteration of the genes for glycophorin A and B in glycophorin-A-deficient individuals.  |  Rahuel, C., et al. 1988. Eur J Biochem. 177: 605-14. PMID: 3197721
    8. Molecular cloning of a human glycophorin B cDNA: nucleotide sequence and genomic relationship to glycophorin A.  |  Siebert, PD. and Fukuda, M. 1987. Proc Natl Acad Sci U S A. 84: 6735-9. PMID: 3477806
    9. Differential enzymatic deglycosylation reveals attachment of red cell B antigen onto the carbohydrate moiety of glycophorin A and glycophorin B.  |  Hsu, K. 2023. Vox Sang. 118: 147-152. PMID: 36510386
    10. A monoclonal antibody directed against the homologous N-terminal domain of glycophorin A and B.  |  Edelman, L., et al. 1984. Exp Clin Immunogenet. 1: 129-39. PMID: 6401002

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Glycophorin A/B Antibody (5F60)

    sc-71158
    100 µg/ml
    $316.00

    Glycophorin A/B Antibody (5F60) FITC

    sc-71158 FITC
    200 µg/ml

    Glycophorin A/B Antibody (5F60) PE

    sc-71158 PE
    100 tests in 2 ml
    $343.00

    What is the recommended storage condition for Glycophorin A/B (5F60): sc-71158?

    Asked by: Professor Griffin
    Thank you for your question. We recommend storing the antibody at 4° C.
    Answered by: Technical Support
    Date published: 2017-03-27
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    Rated 4 out of 5 by from Great Western blot data obtained in KGreat Western blot data obtained in K-562 whole cell lysate. -SCBT QC
    Date published: 2013-02-11
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    Glycophorin A/B Antibody (5F60) is rated 4.0 out of 5 by 1.
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