Glycophorin A Antibody (H-12): sc-166851

Glycophorin A Antibody (H-12) is a mouse monoclonal IgG2b antibody that detects Glycophorin A in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Glycophorin A is a crucial sialoglycoprotein located in the human erythrocyte membrane, where Glycophorin A plays a significant role in maintaining the structural integrity of red blood cells and facilitating cell-cell interactions. Glycophorin A spans the membrane once, with its amino-terminal end exposed to the extracellular environment, which is essential for Glycophorin A′s function in defining blood group phenotypes through antigenic determinants. The Glycophorin A gene, located on chromosome 4q31.21, consists of seven exons and encodes a 150 amino acid protein that is 97% homologous to Glycophorin B, highlighting the evolutionary relationship between these proteins. The presence of Glycophorin A on the erythrocyte surface is vital not only for blood group classification but also for the prevention of hemolytic disease, making Glycophorin A an important target for both clinical diagnostics and research applications. Anti-Glycophorin A antibody (H-12) is an invaluable tool for studying erythrocyte biology and the underlying mechanisms of blood group antigen expression.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Glycophorin A Antibody (H-12) References:

1. Glycophorin A dimerization and band 3 interaction during erythroid membrane biogenesis: in vivo studies in human glycophorin A transgenic mice.  |  Auffray, I., et al. 2001. Blood. 97: 2872-8. PMID: 11313283
2. Interaction of glycophorin A with lectins as measured by surface plasmon resonance (SPR).  |  Krotkiewska, B., et al. 2002. Acta Biochim Pol. 49: 481-90. PMID: 12362990
3. The effect of carboxymethylating a single methionine residue on the subunit interactions of glycophorin A.  |  Silverberg, M., et al. 1976. Biochemistry. 15: 1448-54. PMID: 1259949
4. A malaria invasion receptor, the 175-kilodalton erythrocyte binding antigen of Plasmodium falciparum recognizes the terminal Neu5Ac(alpha 2-3)Gal- sequences of glycophorin A.  |  Orlandi, PA., et al. 1992. J Cell Biol. 116: 901-9. PMID: 1310320
5. Glycophorin A interacts with interleukin-2 and inhibits interleukin-2-dependent T-lymphocyte proliferation.  |  Chu, JW. and Sharom, FJ. 1992. Cell Immunol. 145: 223-39. PMID: 1451177
6. Retrospective assessment of radiation exposure using biological dosimetry: chromosome painting, electron paramagnetic resonance and the glycophorin a mutation assay.  |  Kleinerman, RA., et al. 2006. Radiat Res. 166: 287-302. PMID: 16808614
7. CD71 is selectively and ubiquitously expressed at high levels in erythroid precursors of all maturation stages: a comparative immunochemical study with glycophorin A and hemoglobin A.  |  Dong, HY., et al. 2011. Am J Surg Pathol. 35: 723-32. PMID: 21415701
8. Non-synonymous amino acid alterations in PfEBA-175 modulate the merozoite ligand's ability to interact with host's Glycophorin A receptor.  |  Chowdhury, P., et al. 2020. Infect Genet Evol. 85: 104418. PMID: 32561295
9. Erythrocyte membrane rigidity induced by glycophorin A-ligand interaction. Evidence for a ligand-induced association between glycophorin A and skeletal proteins.  |  Chasis, JA., et al. 1985. J Clin Invest. 75: 1919-26. PMID: 4008645
10. Use of the glycophorin A human mutation assay to study workers exposed to styrene.  |  Compton-Quintana, PJ., et al. 1993. Environ Health Perspect. 99: 297-301. PMID: 8319648

The preferred Glycophorin A antibody is Glycophorin A Antibody (R10): sc-53905.