Glycophorin A Antibody (E4): sc-59181

Glycophorin A Antibody (E4) is a mouse monoclonal IgM antibody that detects Glycophorin A in human samples through applications such as western blotting (WB), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Glycophorin A is a crucial sialoglycoprotein located on the surface of human erythrocytes, where Glycophorin A plays a significant role in maintaining the structural integrity of red blood cells and facilitating their interaction with the surrounding plasma. Glycophorin A spans the erythrocyte membrane once, with its amino-terminal end exposed to the extracellular environment, which is essential for providing a negative charge to the cell surface, thereby preventing red blood cells from aggregating. Glycophorin A is also vital for defining blood group phenotypes, as Glycophorin A carries specific antigenic determinants that are critical for blood transfusion compatibility and immune response. The Glycophorin A gene, located on chromosome 4q31.21, consists of seven exons and encodes a 150 amino acid protein that is 97% homologous to Glycophorin B, highlighting the evolutionary relationship between these proteins. Anti-Glycophorin A antibody (E4) is an invaluable tool for researchers studying erythrocyte biology, blood group antigens, and related hematological disorders.

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Glycophorin A Antibody (E4) References:

1. A novel St(a) glycophorin produced via gene conversion of pseudoexon III from glycophorin E to glycophorin A gene.  |  Huang, CH., et al. 2000. Hum Mutat. 15: 533-40. PMID: 10862083
2. Glycophorin A in two patients with congenital dyserythropoietic anemia type I and type II is partly unglycosylated.  |  Zdebska, E., et al. 2000. Acta Biochim Pol. 47: 773-9. PMID: 11310976
3. Quantification of glycophorin A and glycophorin B on normal human RBCs by flow cytometry.  |  de Isla, NG., et al. 2003. Transfusion. 43: 1145-52. PMID: 12869123
4. Glycophorin A-deficient red cells may have a weak expression of C4-bound Ch and Rg antigens.  |  Tippett, P., et al. 1996. Immunohematology. 12: 4-7. PMID: 15387753
5. Glycophorin A on normal and leukemia cells detected by monoclonal antibodies, including a new monoclonal antibody reactive with glycophorins A and B.  |  Rearden, A., et al. 1985. Mol Immunol. 22: 369-78. PMID: 2412109
6. Crystal Structure of the Glycophorin A Transmembrane Dimer in Lipidic Cubic Phase.  |  Trenker, R., et al. 2015. J Am Chem Soc. 137: 15676-9. PMID: 26642914
7. Glycophorin A interferes in the agglutination of human erythrocytes by concanavalin A. Explanation of the requirement for enzymic predigestion.  |  Gokhale, SM. and Mehta, NG. 1987. Biochem J. 241: 505-11. PMID: 3297033
8. Diagnostic value of glycophorin-A in comparison with P57 immunohistochemical staining method in differentiating complete and partial molar pregnancies.  |  Nili, F., et al. 2021. Ann Diagn Pathol. 53: 151769. PMID: 34146830
9. Human glycophorin A and B are encoded by separate, single copy genes coordinately regulated by a tumor-promoting phorbol ester.  |  Siebert, PD. and Fukuda, M. 1986. J Biol Chem. 261: 12433-6. PMID: 3755722
10. Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia.  |  Andersson, LC., et al. 1979. Int J Cancer. 24: 717-20. PMID: 397196

The preferred Glycophorin A antibody is Glycophorin A Antibody (R10): sc-53905.