Glycophorin A Antibody (E4) recommended for detection of red blood cell Glycophorin A of human origin by WB, IF, IHC(P) and FCM

Glycophorin A Antibody (E4): sc-59181

Glycophorin A Antibody (E4) is rated 4.0 out of 5 by 2.
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Datasheets

    • Glycophorin A Antibody (E4) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • raised against human thymus
    • recommended for detection of red blood cell Glycophorin A of human origin by WB, IF, IHC(P) and FCM; non cross-reactive with glycophorin B and trypsinised cells
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Glycophorin A (R10): sc-53905 for Glycophorin A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

How can Glycophorin A (E4): sc-59181 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Trav11
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-59181 PE) or fluoroscein (sc-59181 FITC).
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Nice band seen in WB using K Nice band seen in WB using K-562 whole cell lysate. -SCBT QC
Date published: 2015-06-28
Rated 4 out of 5 by from Produced decent immunoperoxidase membrane Produced decent immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human blood vessel tissue. -SCBT QC
Date published: 2013-10-25
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