Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
glycogen synthase 2 CRISPR Activation Plasmid (h): sc-402704-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- glycogen synthase 2 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- glycogen synthase 2 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by glycogen synthase 2 CRISPR Activation Plasmid (h) and glycogen synthase 2 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the GYS2 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: glycogen synthase 2 Antibody (G-8): sc-390391
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
glycogen synthase 2 CRISPR Activation Plasmid (h) | sc-402704-ACT | 20 µg | $397.00 |
Human GYS2 encodes glycogen synthase 2, the liver-enriched rate-limiting enzyme that catalyzes elongation of glycogen chains from UDP-glucose, coordinating postprandial glucose storage and systemic glycemic homeostasis. Its activity is tightly regulated by phosphorylation and allosteric activation by glucose-6-phosphate, integrating insulin signaling with nutrient-sensing pathways that control hepatic carbohydrate metabolism. GYS2 function intersects with glycogen metabolism, gluconeogenesis balance, and broader energy homeostasis programs that shape hepatocyte physiology. Genetic or regulatory perturbation of GYS2 has been linked to disordered hepatic glycogen handling and metabolic phenotypes relevant to studies of insulin resistance and related liver-associated metabolic dysfunction.
glycogen synthase 2 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous GYS2 expression without altering the underlying DNA sequence.
glycogen synthase 2 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the GYS2 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the GYS2 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous glycogen synthase 2 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native GYS2 locus and enabling the study of glycogen synthase 2-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of glycogen synthase 2 pathway restoration in tumor cells with silenced or reduced GYS2 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.