Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Glutathione reductase CRISPR/Cas9 KO Plasmid (h): sc-417499
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glutathione reductase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Glutathione reductase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Glutathione reductase Antibody (C-10): sc-133245
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glutathione reductase CRISPR/Cas9 KO Plasmid (h) | sc-417499 | 20 µg | $397.00 |
Overview
GSR encodes human glutathione reductase, a FAD-dependent oxidoreductase that regenerates reduced glutathione (GSH) from oxidized glutathione (GSSG) using NADPH, maintaining intracellular redox homeostasis. By sustaining the GSH pool, GSR supports detoxification of reactive oxygen species, preserves thiol status of proteins, and modulates redox-sensitive signaling across cytosolic and mitochondrial compartments. This activity interfaces with NADPH-producing pathways such as the pentose phosphate pathway and contributes to cellular defenses during oxidative, metabolic, and inflammatory stress. Altered GSR function or expression is commonly studied in contexts of redox imbalance, including hemolytic phenotypes, neurodegenerative processes, and cancer cell oxidative stress adaptation.
Glutathione reductase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GSR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GSR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GSR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glutathione reductase protein expression.
This CRISPR knockout system enables efficient generation of GSR-deficient cell models for investigation of Glutathione reductase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GSR exon(s) critical for Glutathione reductase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GSR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Glutathione reductase CRISPR/Cas9 KO Plasmid (h) and Glutathione reductase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GSR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Glutathione reductase HDR Plasmid (h) and Glutathione reductase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GSR homology arms to support homology-directed repair at defined GSR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.