Glutathione Peroxidase 3/GPX3 CRISPR/Cas9 KO Plasmid (m): sc-420664

Overview

Glutathione peroxidase 3 (Gpx3/GPX3) is a secreted selenoenzyme that reduces hydrogen peroxide and organic hydroperoxides using glutathione, contributing to extracellular redox homeostasis and limiting oxidative damage to proteins and lipids. In mouse tissues and plasma, GPX3 helps buffer reactive oxygen species and interfaces functionally with glutathione metabolism, antioxidant defense networks, and inflammation-associated signaling. Altered GPX3 activity has been linked in the literature to oxidative stress phenotypes and dysregulated tissue remodeling, with downstream effects on endothelial function, immune cell activation, and redox-sensitive transcriptional programs. Because extracellular peroxide tone influences pathways such as MAPK and NF-κB, Gpx3 perturbation is frequently used to interrogate redox-regulated signaling and oxidative injury models.

Glutathione Peroxidase 3/GPX3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gpx3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gpx3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gpx3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glutathione Peroxidase 3/GPX3 protein expression.

This CRISPR knockout system enables efficient generation of Gpx3-deficient cell models for investigation of Glutathione Peroxidase 3/GPX3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Gpx3 exon(s) critical for Glutathione Peroxidase 3/GPX3 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Gpx3 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Glutathione Peroxidase 3/GPX3 CRISPR/Cas9 KO Plasmid (m) and Glutathione Peroxidase 3/GPX3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gpx3 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Glutathione Peroxidase 3/GPX3 HDR Plasmid (m) and Glutathione Peroxidase 3/GPX3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gpx3 homology arms to support homology-directed repair at defined Gpx3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.