Glutathione Peroxidase 1/GPX1 CRISPR/Cas9 KO Plasmid (h): sc-400631

Overview

GPX1 encodes the cytosolic selenoenzyme glutathione peroxidase 1, a major antioxidant defense protein that reduces hydrogen peroxide and lipid hydroperoxides using glutathione as an electron donor. By limiting reactive oxygen species accumulation, GPX1 helps maintain redox homeostasis and influences signaling networks linked to oxidative stress responses, including NRF2-regulated programs, mitochondrial function, and inflammatory pathways. Altered GPX1 activity has been associated with mechanisms relevant to cancer biology, metabolic dysfunction, neurodegeneration, and cardiovascular pathology, where redox imbalance can reshape cell survival and stress-adaptation phenotypes. As a broadly expressed enzyme, GPX1 is frequently used to probe how antioxidant capacity modulates DNA damage responses, ferroptosis sensitivity, and cellular responses to hypoxia or inflammatory cues.

Glutathione Peroxidase 1/GPX1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPX1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPX1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPX1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glutathione Peroxidase 1/GPX1 protein expression.

This CRISPR knockout system enables efficient generation of GPX1-deficient cell models for investigation of Glutathione Peroxidase 1/GPX1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting GPX1 exon(s) critical for Glutathione Peroxidase 1/GPX1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple GPX1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Glutathione Peroxidase 1/GPX1 CRISPR/Cas9 KO Plasmid (h) and Glutathione Peroxidase 1/GPX1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPX1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Glutathione Peroxidase 1/GPX1 HDR Plasmid (h) and Glutathione Peroxidase 1/GPX1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPX1 homology arms to support homology-directed repair at defined GPX1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.