Glut9 CRISPR/Cas9 KO Plasmid (h): sc-406232
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glut9 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Glut9 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glut9 CRISPR/Cas9 KO Plasmid (h) | sc-406232 | 20 µg | $397.00 |
Overview
SLC2A9 encodes Glut9, a facilitative transporter that plays a central role in cellular urate handling and hexose transport, with prominent function in renal and hepatic physiology. Glut9 contributes to transmembrane movement of uric acid and related metabolites, influencing systemic urate homeostasis and redox balance. Altered SLC2A9 activity is linked to variation in serum urate levels and has been implicated in disorders of purine metabolism, including hyperuricemia and gout susceptibility, as well as kidney-related phenotypes. In cell models, SLC2A9 perturbation can affect metabolic flux, oxidative stress responses, and transporter networks relevant to epithelial transport and nutrient sensing pathways.
Glut9 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC2A9 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC2A9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC2A9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glut9 protein expression.
This CRISPR knockout system enables efficient generation of SLC2A9-deficient cell models for investigation of Glut9 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC2A9 exon(s) critical for Glut9 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC2A9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Glut9 CRISPR/Cas9 KO Plasmid (h) and Glut9 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC2A9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Glut9 HDR Plasmid (h) and Glut9 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC2A9 homology arms to support homology-directed repair at defined SLC2A9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.