Glut12 CRISPR/Cas9 KO Plasmid (m): sc-436139
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glut12 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Glut12 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glut12 CRISPR/Cas9 KO Plasmid (m) | sc-436139 | 20 µg | $397.00 |
Overview
Slc2a12 encodes glucose transporter 12 (GLUT12), a facilitative hexose transporter that supports basal and insulin-responsive glucose uptake depending on cell type and metabolic state. In mouse tissues, GLUT12 contributes to cellular glucose homeostasis and integrates with pathways controlling energy utilization, including glycolysis, glycogen metabolism, and insulin/PI3K–AKT signaling. Altered Slc2a12 expression or trafficking has been associated with metabolic adaptation in insulin-sensitive tissues and may influence phenotypes linked to obesity, insulin resistance, and related cardiometabolic stress responses. Studying Glut12 function helps clarify how distinct GLUT family members partition glucose flux across tissues and how transporter redundancy shapes metabolic resilience.
Glut12 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc2a12 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc2a12 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc2a12 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glut12 protein expression.
This CRISPR knockout system enables efficient generation of Slc2a12-deficient cell models for investigation of Glut12 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slc2a12 exon(s) critical for Glut12 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slc2a12 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Glut12 CRISPR/Cas9 KO Plasmid (m) and Glut12 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc2a12 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Glut12 HDR Plasmid (m) and Glut12 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc2a12 homology arms to support homology-directed repair at defined Slc2a12 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.