Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GLUD1 CRISPR/Cas9 KO Plasmid (h): sc-402187
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GLUD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GLUD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GLUD1 CRISPR/Cas9 KO Plasmid (h) | sc-402187 | 20 µg | $397.00 |
Overview
GLUD1 encodes mitochondrial glutamate dehydrogenase 1 (GDH1), an NAD(P)+-dependent enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and ammonia, linking amino acid metabolism to the TCA cycle and cellular redox balance. By controlling the flux between glutaminolysis and oxidative metabolism, GLUD1 influences mitochondrial bioenergetics, nitrogen handling, and anaplerotic replenishment of central carbon metabolism. Altered GDH1 activity has been associated with metabolic dysregulation affecting insulin secretion and excitatory neurotransmitter homeostasis, making GLUD1 a relevant target in studies of mitochondrial metabolism and neuroendocrine signaling.
GLUD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GLUD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GLUD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GLUD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GLUD1 protein expression.
This CRISPR knockout system enables efficient generation of GLUD1-deficient cell models for investigation of GLUD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GLUD1 exon(s) critical for GLUD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GLUD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GLUD1 CRISPR/Cas9 KO Plasmid (h) and GLUD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GLUD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GLUD1 HDR Plasmid (h) and GLUD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GLUD1 homology arms to support homology-directed repair at defined GLUD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.