Glucosidase IIβ Antibody (H-4): sc-374457
- Glucosidase IIβ Antibody (H-4) is a mouse monoclonal IgG2b κ Glucosidase IIβ antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 9-41 near the N-terminus of Glucosidase IIβ of human origin
- Glucosidase II beta Antibody (H-4) is recommended for detection of the β subunit of Glucosidase II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine
- Anti-Glucosidase II beta Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for Glucosidase IIβ Antibody (H-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with Glucosidase IIβ Antibody (H-4) (see ordering information below).
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Glucosidase IIβ Antibody (H-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Glucosidase II beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Glucosidase IIβ (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glucosidase II beta protein plays a crucial role in processing N-linked glycoproteins within the endoplasmic reticulum, where Glucosidase II beta protein is involved in trimming glucose residues from glycan chains. This process is essential for proper protein folding and quality control, as misfolded proteins are typically targeted for degradation. Glucosidase II beta protein forms a heterodimeric complex with Glucosidase IIα, which serves as the catalytic core. Glucosidase II beta protein is rich in glutamic and aspartic acid residues and contains a putative endoplasmic reticulum retention signal (HDEL) at its C-terminus, ensuring localization within the ER. The phosphorylated form of Glucosidase II beta protein is found in the plasma membrane and is highly expressed in fibroblasts stimulated by fibroblast growth factor and in epidermal carcinoma cells. Initially purified from a human carcinoma cell line, Glucosidase II beta protein has been identified as a potential substrate for protein kinase C, highlighting Glucosidase II beta protein′s significance in cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Glucosidase IIβ Antibody (H-4) References:
- The alpha- and beta-subunits are required for expression of catalytic activity in the hetero-dimeric glucosidase II complex from human liver. | Treml, K., et al. 2000. Glycobiology. 10: 493-502. PMID: 10764838
- Quaternary and domain structure of glycoprotein processing glucosidase II. | Trombetta, ES., et al. 2001. Biochemistry. 40: 10717-22. PMID: 11524018
- Purification and characterization of glucosidase II involved in N-linked glycoprotein processing in bovine mammary gland. | Saxena, S., et al. 1987. Biochem J. 247: 563-70. PMID: 3122729
- Purification and characterization of glucosidase I involved in N-linked glycoprotein processing in bovine mammary gland. | Shailubhai, K., et al. 1987. Biochem J. 247: 555-62. PMID: 3322267
- Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, conserved from yeast to mammals, and a tightly bound noncatalytic HDEL-containing subunit. | Trombetta, ES., et al. 1996. J Biol Chem. 271: 27509-16. PMID: 8910335
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glucosidase IIβ Antibody (H-4) | sc-374457 | 200 µg/ml | $316.00 | |||
Glucosidase IIβ Antibody (H-4): m-IgG2b BP-HRP Bundle | sc-548740 | 200 µg Ab; 10 µg BP | $354.00 | |||
Glucosidase IIβ Antibody (H-4) AC | sc-374457 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Glucosidase IIβ Antibody (H-4) HRP | sc-374457 HRP | 200 µg/ml | $316.00 | |||
Glucosidase IIβ Antibody (H-4) FITC | sc-374457 FITC | 200 µg/ml | $330.00 | |||
Glucosidase IIβ Antibody (H-4) PE | sc-374457 PE | 200 µg/ml | $343.00 | |||
Glucosidase IIβ Antibody (H-4) Alexa Fluor® 488 | sc-374457 AF488 | 200 µg/ml | $357.00 | |||
Glucosidase IIβ Antibody (H-4) Alexa Fluor® 546 | sc-374457 AF546 | 200 µg/ml | $357.00 | |||
Glucosidase IIβ Antibody (H-4) Alexa Fluor® 594 | sc-374457 AF594 | 200 µg/ml | $357.00 | |||
Glucosidase IIβ Antibody (H-4) Alexa Fluor® 647 | sc-374457 AF647 | 200 µg/ml | $357.00 | |||
Glucosidase IIβ Antibody (H-4) Alexa Fluor® 680 | sc-374457 AF680 | 200 µg/ml | $357.00 | |||
Glucosidase IIβ Antibody (H-4) Alexa Fluor® 790 | sc-374457 AF790 | 200 µg/ml | $357.00 | |||
Glucosidase IIβ (H-4) Neutralizing Peptide | sc-374457 P | 100 µg/0.5 ml | $68.00 |