Glucose Transporter Glut5 CRISPR/Cas9 KO Plasmid (m): sc-425193

Overview

Slc2a5 encodes glucose transporter Glut5 (GLUT5), a facilitative hexose transporter with high specificity for fructose that mediates apical fructose uptake and contributes to carbohydrate handling in intestinal epithelium and other tissues. By regulating fructose influx, GLUT5 supports cellular energy metabolism and influences glycolytic flux and downstream lipid synthesis pathways, linking nutrient availability to metabolic remodeling. Altered SLC2A5/GLUT5 expression has been associated with diet-responsive metabolic phenotypes and is frequently examined in contexts of intestinal absorption, hepatic metabolism, and tumor cell nutrient utilization. In mouse models, Slc2a5 serves as a tractable node for studying how fructose transport shapes systemic energy balance and microenvironmental metabolic stress.

Glucose Transporter Glut5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc2a5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc2a5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc2a5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glucose Transporter Glut5 protein expression.

This CRISPR knockout system enables efficient generation of Slc2a5-deficient cell models for investigation of Glucose Transporter Glut5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Slc2a5 exon(s) critical for Glucose Transporter Glut5 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Slc2a5 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Glucose Transporter Glut5 CRISPR/Cas9 KO Plasmid (m) and Glucose Transporter Glut5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc2a5 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Glucose Transporter Glut5 HDR Plasmid (m) and Glucose Transporter Glut5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc2a5 homology arms to support homology-directed repair at defined Slc2a5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.