Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Glucose Transporter Glut3 CRISPR/Cas9 KO Plasmid (m): sc-423000
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glucose Transporter Glut3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Glucose Transporter Glut3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Glucose Transporter Glut3 Antibody (G-5): sc-74399
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glucose Transporter Glut3 CRISPR/Cas9 KO Plasmid (m) | sc-423000 | 20 µg | $397.00 |
Overview
Slc2a3 encodes the high-affinity facilitative glucose transporter GLUT3, a major route for basal glucose uptake in mouse cells with elevated metabolic demand, including neurons and activated immune populations. By regulating intracellular glucose availability, GLUT3 supports glycolytic flux, ATP production, and biosynthetic pathways that couple nutrient sensing to cell survival and proliferation. Altered GLUT3 activity can shift redox balance and metabolic signaling networks such as AMPK and mTOR, impacting stress responses and differentiation programs. Dysregulated glucose transport is frequently examined in contexts of neurobiology, inflammation, and metabolically driven disease phenotypes where changes in glucose utilization contribute to pathophysiology.
Glucose Transporter Glut3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc2a3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc2a3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc2a3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glucose Transporter Glut3 protein expression.
This CRISPR knockout system enables efficient generation of Slc2a3-deficient cell models for investigation of Glucose Transporter Glut3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slc2a3 exon(s) critical for Glucose Transporter Glut3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slc2a3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Glucose Transporter Glut3 CRISPR/Cas9 KO Plasmid (m) and Glucose Transporter Glut3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc2a3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Glucose Transporter Glut3 HDR Plasmid (m) and Glucose Transporter Glut3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc2a3 homology arms to support homology-directed repair at defined Slc2a3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.