GLG1 CRISPR/Cas9 KO Plasmid (m): sc-422869
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GLG1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GLG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GLG1 CRISPR/Cas9 KO Plasmid (m) | sc-422869 | 20 µg | $397.00 |
Overview
Glg1 encodes GLG1, a Golgi-associated glycoprotein implicated in protein sorting and trafficking within the secretory pathway, supporting proper cargo processing and localization across the endomembrane system. Through its Golgi residency and luminal glycosylation context, GLG1 contributes to regulated transport of proteins that influence cell–cell interactions and extracellular matrix communication. Altered Golgi organization and glycoprotein processing are frequently linked to dysregulated adhesion, migration, and stress responses, making Glg1 a useful node for investigating how secretory pathway perturbations reshape cellular phenotypes. In mouse models and primary cell systems, Glg1 perturbation can be leveraged to study pathway-level consequences of Golgi dysfunction on signaling output and proteostasis.
GLG1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Glg1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Glg1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Glg1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GLG1 protein expression.
This CRISPR knockout system enables efficient generation of Glg1-deficient cell models for investigation of GLG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Glg1 exon(s) critical for GLG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Glg1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GLG1 CRISPR/Cas9 KO Plasmid (m) and GLG1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Glg1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GLG1 HDR Plasmid (m) and GLG1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Glg1 homology arms to support homology-directed repair at defined Glg1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.