Gle1 Antibody (G-9): sc-514796

Gle1 Antibody (G-9) is a mouse monoclonal IgG1 antibody that detects Gle1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gle1 (G-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gle1 protein, also known as GLE1L, is a crucial component in the transport of poly(A)-containing mRNAs from the nucleus to the cytoplasm, a process essential for gene expression regulation. This 698 amino acid protein is expressed in two alternatively spliced isoforms and is localized to both the nucleus and cytoplasm, where Gle1 associates with the nuclear pore complex (NPC). Proper functioning of Gle1 is vital, as defects in the gene encoding Gle1 can lead to severe developmental disorders, including lethal congenital contracture syndrome type 1 (LCCS1) and lethal arthrogryposis with anterior horn cell disease (LAAHD). These conditions are characterized by significant prenatal complications, such as hydrops and respiratory failure, underscoring the importance of Gle1 in normal cellular function and development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Gle1 Antibody (G-9) References:

1. An essential role for hGle1 nucleocytoplasmic shuttling in mRNA export.  |  Kendirgi, F., et al. 2003. J Cell Biol. 160: 1029-40. PMID: 12668658
2. The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155.  |  Rayala, HJ., et al. 2004. Mol Cell Proteomics. 3: 145-55. PMID: 14645504
3. Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: a conserved mechanism in the export of Hsp70 mRNA.  |  Kendirgi, F., et al. 2005. Mol Biol Cell. 16: 4304-15. PMID: 16000379
4. Mutations in mRNA export mediator GLE1 result in a fetal motoneuron disease.  |  Nousiainen, HO., et al. 2008. Nat Genet. 40: 155-7. PMID: 18204449
5. Gle1 does double duty.  |  Kutay, U. and Panse, VG. 2008. Cell. 134: 564-6. PMID: 18724928
6. The mRNA export factor Gle1 and inositol hexakisphosphate regulate distinct stages of translation.  |  Bolger, TA., et al. 2008. Cell. 134: 624-33. PMID: 18724935
7. Gle1 functions during mRNA export in an oligomeric complex that is altered in human disease.  |  Folkmann, AW., et al. 2013. Cell. 155: 582-93. PMID: 24243016
8. Insights into mRNA export-linked molecular mechanisms of human disease through a Gle1 structure-function analysis.  |  Folkmann, AW., et al. 2014. Adv Biol Regul. 54: 74-91. PMID: 24275432
9. Functions of Gle1 are governed by two distinct modes of self-association.  |  Mason, AC. and Wente, SR. 2020. J Biol Chem. 295: 16813-16825. PMID: 32981894
10. The nucleoporin Gle1 activates DEAD-box protein 5 (Dbp5) by promoting ATP binding and accelerating rate limiting phosphate release.  |  Gray, S., et al. 2022. Nucleic Acids Res. 50: 3998-4011. PMID: 35286399
11. Phosphorylation impacts GLE1 nuclear localization and association with DDX1.  |  Sharma, M., et al. 2023. Adv Biol Regul. 90: 100990. PMID: 37801910
12. The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export.  |  Watkins, JL., et al. 1998. Proc Natl Acad Sci U S A. 95: 6779-84. PMID: 9618489

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Gle1 Antibody (G-9) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Gle1 Antibody (G-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Gle1 Antibody (G-9) Specifications

Alternate Product Name: Gle1

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ lambda light chain

Amino Acids: 399-698

Epitope Target: Gle1

Epitope Species: human

Product Reactivity/Detection: Gle1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Gle1 Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin