Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GLDC Double Nickase Plasmid (h): sc-403511-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GLDC Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GLDC Double Nickase Plasmid (h) and GLDC Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GLDC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GLDC Antibody (H-9): sc-376196
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GLDC Double Nickase Plasmid (h) | sc-403511-NIC | 20 µg | $410.00 | |||
GLDC Double Nickase Plasmid (h2) | sc-403511-NIC-2 | 20 µg | $410.00 |
GLDC encodes glycine decarboxylase, the P-protein component of the mitochondrial glycine cleavage system that catalyzes glycine decarboxylation and transfers the resulting one-carbon unit to tetrahydrofolate. Through this reaction, GLDC links glycine catabolism to folate-mediated one-carbon metabolism, influencing mitochondrial redox balance, nucleotide biosynthesis, and cellular methylation capacity. GLDC activity integrates with amino acid homeostasis and mitochondrial energy pathways by contributing NADH generation and regulating glycine-derived carbon flux. Dysregulation or loss of GLDC function is associated with altered glycine handling and perturbations in one-carbon metabolism pathways relevant to inborn errors of metabolism and context-dependent metabolic dependencies in disease models.
GLDC Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GLDC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GLDC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GLDC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GLDC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.