GILT Antibody (G-11): sc-393507
- GILT Antibody (G-11) is a mouse monoclonal IgG1 κ GILT antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 146-171 within an internal region of GILT of human origin
- GILT Antibody (G-11) is recommended for detection of GILT precursor and mature chains of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GILT Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GILT Antibody (G-11) for WB applications. These reagents are now offered in bundles with GILT Antibody (G-11) (see ordering information below).
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SEE ALSO...
GILT Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects GILT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GILT (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GILT protein plays a crucial role in the endocytic pathway, where GILT facilitates the reduction of disulfide bonds in proteins that are internalized and subsequently degraded. This reduction is essential for the generation of major histocompatibility complex (MHC) class II-peptide complexes, which are vital for the immune response. GILT acts as a gamma-interferon-inducible thiol reductase, optimally functioning at low pH within lysosomes, and is constitutively expressed in antigen-presenting cells while being induced in other cell types by gamma-interferon. The processing of GILT from its precursor form to its mature state occurs in MHC class II-containing compartments, highlighting GILT′s importance in antigen processing and presentation. Notably, GILT expression in tumor cells is critical for T cell recognition of specific epitopes, and GILT absence in melanomas can significantly alter antigen processing and the hierarchy of immunodominant epitope presentation, underscoring GILT′s potential impact on immune responses in cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GILT Antibody (G-11) References:
- Thiol oxidation and reduction in MHC-restricted antigen processing and presentation. | Cresswell, P., et al. 1999. Immunol Res. 19: 191-200. PMID: 10493173
- Enzymatic reduction of disulfide bonds in lysosomes: characterization of a gamma-interferon-inducible lysosomal thiol reductase (GILT). | Arunachalam, B., et al. 2000. Proc Natl Acad Sci U S A. 97: 745-50. PMID: 10639150
- Gamma-interferon-inducible lysosomal thiol reductase (GILT). Maturation, activity, and mechanism of action. | Phan, UT., et al. 2000. J Biol Chem. 275: 25907-14. PMID: 10852914
- Absence of gamma-interferon-inducible lysosomal thiol reductase in melanomas disrupts T cell recognition of select immunodominant epitopes. | Haque, MA., et al. 2002. J Exp Med. 195: 1267-77. PMID: 12021307
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GILT Antibody (G-11) | sc-393507 | 200 µg/ml | $322.00 | |||
GILT Antibody (G-11): m-IgG Fc BP-HRP Bundle | sc-530472 | 200 µg Ab; 10 µg BP | $361.00 | |||
GILT Antibody (G-11): m-IgGκ BP-HRP Bundle | sc-523969 | 200 µg Ab, 40 µg BP | $361.00 | |||
GILT Antibody (G-11): m-IgG1 BP-HRP Bundle | sc-544070 | 200 µg Ab; 20 µg BP | $361.00 | |||
GILT Antibody (G-11) AC | sc-393507 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GILT Antibody (G-11) HRP | sc-393507 HRP | 200 µg/ml | $322.00 | |||
GILT Antibody (G-11) FITC | sc-393507 FITC | 200 µg/ml | $336.00 | |||
GILT Antibody (G-11) PE | sc-393507 PE | 200 µg/ml | $349.00 | |||
GILT Antibody (G-11) Alexa Fluor® 488 | sc-393507 AF488 | 200 µg/ml | $364.00 | |||
GILT Antibody (G-11) Alexa Fluor® 546 | sc-393507 AF546 | 200 µg/ml | $364.00 | |||
GILT Antibody (G-11) Alexa Fluor® 594 | sc-393507 AF594 | 200 µg/ml | $364.00 | |||
GILT Antibody (G-11) Alexa Fluor® 647 | sc-393507 AF647 | 200 µg/ml | $364.00 | |||
GILT Antibody (G-11) Alexa Fluor® 680 | sc-393507 AF680 | 200 µg/ml | $364.00 | |||
GILT Antibody (G-11) Alexa Fluor® 790 | sc-393507 AF790 | 200 µg/ml | $364.00 | |||
GILT (G-11) Neutralizing Peptide | sc-393507 P | 100 µg/0.5 ml | $69.00 |