GIF Antibody (D-6): sc-514523

GIF Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects GIF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GIF Antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GIF protein, or gastric intrinsic factor, is a 417 amino acid secreted glycoprotein produced by the parietal cells of the stomach, playing a crucial role in vitamin B12 absorption. When vitamin B12 binds to GIF protein, absorption occurs in the terminal ileum through the receptor cubilin, which is essential for maintaining adequate nutrient levels. Vitamin B12 is necessary for red blood cell formation and proper nervous system functioning, as this vitamin participates in DNA component synthesis, particularly thymine. GIF deficiency can lead to pernicious anemia, an autoimmune condition destroying parietal cells and causing vitamin B12 malabsorption, resulting in megaloblastic anemia characterized by large, immature, and dysfunctional red blood cells. Hereditary intrinsic factor deficiency, caused by GIF gene defects, also leads to megaloblastic anemia, highlighting GIF protein′s importance in overall health and nutrient absorption.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GIF Antibody (D-6) References:

1. Cellular import of cobalamin (Vitamin B-12).  |  Seetharam, B., et al. 1999. J Nutr. 129: 1761-4. PMID: 10498744
2. Metal-thiolate clusters in neuronal growth inhibitory factor (GIF).  |  Vasák, M., et al. 2000. J Inorg Biochem. 79: 7-10. PMID: 10830840
3. The gif paradox.  |  Stavropoulos, P., et al. 2001. Acc Chem Res. 34: 745-52. PMID: 11560474
4. Cobalamin transport proteins and their cell-surface receptors.  |  Seetharam, B. and Yammani, RR. 2003. Expert Rev Mol Med. 5: 1-18. PMID: 14585166
5. A genetic polymorphism in the coding region of the gastric intrinsic factor gene (GIF) is associated with congenital intrinsic factor deficiency.  |  Gordon, MM., et al. 2004. Hum Mutat. 23: 85-91. PMID: 14695536
6. Hereditary juvenile cobalamin deficiency caused by mutations in the intrinsic factor gene.  |  Tanner, SM., et al. 2005. Proc Natl Acad Sci U S A. 102: 4130-3. PMID: 15738392
7. TIFF, GIF, and PNG: get the picture?  |  Kabachinski, J. 2007. Biomed Instrum Technol. 41: 297-300. PMID: 17849757
8. [Vitamin B12 deficiency: a challenging diagnosis and treatment].  |  Rufenacht, P., et al. 2008. Rev Med Suisse. 4: 2212-4, 2216-7. PMID: 19024576
9. Pernicious anemia: new insights from a gastroenterological point of view.  |  Lahner, E. and Annibale, B. 2009. World J Gastroenterol. 15: 5121-8. PMID: 19891010
10. Human gastric intrinsic factor: characterization of cDNA and genomic clones and localization to human chromosome 11.  |  Hewitt, JE., et al. 1991. Genomics. 10: 432-40. PMID: 2071148
11. GIF-2209, an Oxindole Derivative, Accelerates Melanogenesis and Melanosome Secretion via the Modification of Lysosomes in B16F10 Mouse Melanoma Cells.  |  Watanabe, M., et al. 2021. Molecules. 27: PMID: 35011407
12. The Rice miR396-GRF-GIF-SWI/SNF Module: A Player in GA Signaling.  |  Lu, Y., et al. 2021. Front Plant Sci. 12: 786641. PMID: 35087553

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GIF Antibody (D-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-GIF Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GIF Antibody (D-6) Specifications

Alternate Product Name: GIF

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 392-411

Epitope Target: GIF

Epitope Species: human

Product Reactivity/Detection: GIF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for GIF Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin