GH-2 CRISPR/Cas9 KO Plasmid (h): sc-401171
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GH-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GH-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GH-2 CRISPR/Cas9 KO Plasmid (h) | sc-401171 | 20 µg | $397.00 |
Overview
Human GH2 encodes growth hormone 2 (GH-2), a pituitary-derived peptide hormone that engages growth hormone receptor signaling to regulate somatic growth, nutrient partitioning, and metabolic homeostasis. Downstream pathway activity includes JAK2/STAT transcriptional programs with cross-talk to IGF axis regulation, influencing cell proliferation, differentiation, and tissue remodeling. Altered growth hormone signaling has been associated with endocrine and metabolic phenotypes and is relevant for studying dysregulated growth control in disease-linked contexts.
GH-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GH2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GH2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GH2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GH-2 protein expression.
This CRISPR knockout system enables efficient generation of GH2-deficient cell models for investigation of GH-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GH2 exon(s) critical for GH-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GH2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GH-2 CRISPR/Cas9 KO Plasmid (h) and GH-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GH2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GH-2 HDR Plasmid (h) and GH-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GH2 homology arms to support homology-directed repair at defined GH2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.