GGTase-Iβ Antibody (D-11): sc-376854
- GGTase-Iβ Antibody (D-11) is a mouse monoclonal IgG2b κ GGTase-Iβ antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-220 mapping at the N-terminus of GGTase-Iβ of human origin
- GGTase-I beta Antibody (D-11) is recommended for detection of GGTase-Iβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine
- Anti-GGTase-I beta Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for GGTase-Iβ Antibody (D-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with GGTase-Iβ Antibody (D-11) (see ordering information below).
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GGTase-Iβ Antibody (D-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects GGTase-I beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GGTase-Iβ (D-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GGTase-I beta protein plays a crucial role in post-translational modification of proteins through geranylgeranylation, which is essential for proper functioning of several signaling pathways, including TGF-β1 signaling pathway. This modification occurs at C-terminal cysteine residues of target proteins containing a CAAX motif, where "A" represents an aliphatic amino acid and "X" is typically leucine. Geranylgeranylation process is vital for activation of Rab GTPases, which are key regulators of intracellular transport and membrane trafficking. By facilitating attachment of a 20-carbon geranylgeranyl group, GGTase-I ensures proteins are correctly localized within cells, thereby influencing various cellular processes such as growth, differentiation, and apoptosis. GGTase-I′s importance in these pathways highlights potential as a therapeutic target in diseases where these processes are dysregulated.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GGTase-Iβ Antibody (D-11) References:
- Stereochemical analysis of the reaction catalyzed by human protein geranylgeranyl transferase. | Clausen, VA., et al. 2001. Biochemistry. 40: 3920-30. PMID: 11300771
- Expression of mammalian geranylgeranyltransferase type-II in Escherichia coli and its application for in vitro prenylation of Rab proteins. | Kalinin, A., et al. 2001. Protein Expr Purif. 22: 84-91. PMID: 11388804
- Phosphoisoprenoids modulate association of Rab geranylgeranyltransferase with REP-1. | Thomä, NH., et al. 2001. J Biol Chem. 276: 48637-43. PMID: 11675392
- Protein prenylation: genes, enzymes, targets, and functions. | Schafer, WR. and Rine, J. 1992. Annu Rev Genet. 26: 209-37. PMID: 1482112
- Thematic review series: lipid posttranslational modifications. Structural biology of protein farnesyltransferase and geranylgeranyltransferase type I. | Lane, KT. and Beese, LS. 2006. J Lipid Res. 47: 681-99. PMID: 16477080
- cDNA cloning and chromosomal localization of the genes encoding the alpha- and beta-subunits of human Rab geranylgeranyl transferase: the 3' end of the alpha-subunit gene overlaps with the transglutaminase 1 gene promoter. | van Bokhoven, H., et al. 1996. Genomics. 38: 133-40. PMID: 8954794
- Single prenyl-binding site on protein prenyl transferases. | Desnoyers, L. and Seabra, MC. 1998. Proc Natl Acad Sci U S A. 95: 12266-70. PMID: 9770475
- Requirement for geranylgeranyl transferase I and acyl transferase in the TGF-beta-stimulated pathway leading to elastin mRNA stabilization. | Kucich, U., et al. 1998. Biochem Biophys Res Commun. 252: 111-6. PMID: 9813154
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GGTase-Iβ Antibody (D-11) | sc-376854 | 200 µg/ml | $322.00 | |||
GGTase-Iβ Antibody (D-11): m-IgG2b BP-HRP Bundle | sc-548757 | 200 µg Ab; 10 µg BP | $361.00 | |||
GGTase-Iβ Antibody (D-11) AC | sc-376854 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GGTase-Iβ Antibody (D-11) HRP | sc-376854 HRP | 200 µg/ml | $322.00 | |||
GGTase-Iβ Antibody (D-11) FITC | sc-376854 FITC | 200 µg/ml | $336.00 | |||
GGTase-Iβ Antibody (D-11) PE | sc-376854 PE | 200 µg/ml | $349.00 | |||
GGTase-Iβ Antibody (D-11) Alexa Fluor® 488 | sc-376854 AF488 | 200 µg/ml | $364.00 | |||
GGTase-Iβ Antibody (D-11) Alexa Fluor® 546 | sc-376854 AF546 | 200 µg/ml | $364.00 | |||
GGTase-Iβ Antibody (D-11) Alexa Fluor® 594 | sc-376854 AF594 | 200 µg/ml | $364.00 | |||
GGTase-Iβ Antibody (D-11) Alexa Fluor® 647 | sc-376854 AF647 | 200 µg/ml | $364.00 | |||
GGTase-Iβ Antibody (D-11) Alexa Fluor® 680 | sc-376854 AF680 | 200 µg/ml | $364.00 | |||
GGTase-Iβ Antibody (D-11) Alexa Fluor® 790 | sc-376854 AF790 | 200 µg/ml | $364.00 |