Gfi-1 Antibody (B-9): sc-376949

Gfi-1 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Gfi-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Gfi-1 antibody (B-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Growth Factor Independent 1 (Gfi-1) plays a crucial role as a transcriptional repressor, specifically binding to the DNA consensus sequence TAAATCAC(A/T)GCA, which is vital for regulating gene expression involved in cell survival and differentiation. Gfi-1′s structure includes six C2H2-type zinc finger motifs located at the carboxy-terminus, with zinc fingers 3, 4, and 5 being essential for DNA binding capability. Gfi-1 contains a 20 amino acid SNAG domain that is critical for transcriptional repression function, particularly in inhibiting the pro-apoptotic gene Bax at both the mRNA and protein levels, thereby preventing cell death. Gfi-1 is predominantly expressed in granulocytes and activated macrophages, extending influence beyond the lymphoid system, while Gfi-1B is primarily found in the bone marrow and spleen, where Gfi-1B also functions as a transcriptional repressor. The genes encoding human Gfi-1 and Gfi-1B are located on chromosomes 1p22 and 9q34.3, respectively, highlighting their genetic significance in cellular processes.

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Gfi-1 Antibody (B-9) References:

1. Regulation of Socs gene expression by the proto-oncoprotein GFI-1B: two routes for STAT5 target gene induction by erythropoietin.  |  Jegalian, AG. and Wu, H. 2002. J Biol Chem. 277: 2345-52. PMID: 11696536
2. Inflammatory reactions and severe neutropenia in mice lacking the transcriptional repressor Gfi1.  |  Karsunky, H., et al. 2002. Nat Genet. 30: 295-300. PMID: 11810106
3. Transcription factor Gfi-1 induced by G-CSF is a negative regulator of CXCR4 in myeloid cells.  |  De La Luz Sierra, M., et al. 2007. Blood. 110: 2276-85. PMID: 17596540
4. Gfi-1 inhibits proliferation and colony formation of p210BCR/ABL-expressing cells via transcriptional repression of STAT 5 and Mcl-1.  |  Soliera, AR., et al. 2012. Leukemia. 26: 1555-63. PMID: 22285998
5. Sudden infant death syndrome: exposure to cigarette smoke leads to hypomethylation upstream of the growth factor independent 1 (GFI1) gene promoter.  |  Schwender, K., et al. 2016. Forensic Sci Med Pathol. 12: 399-406. PMID: 27677632
6. Chromosomal localization of a gene, GF1, encoding a novel zinc finger protein reveals a new syntenic region between man and rodents.  |  Bell, DW., et al. 1995. Cytogenet Cell Genet. 70: 263-7. PMID: 7789186
7. Progression of interleukin-2 (IL-2)-dependent rat T cell lymphoma lines to IL-2-independent growth following activation of a gene (Gfi-1) encoding a novel zinc finger protein.  |  Gilks, CB., et al. 1993. Mol Cell Biol. 13: 1759-68. PMID: 8441411
8. Gfi-1 encodes a nuclear zinc finger protein that binds DNA and functions as a transcriptional repressor.  |  Zweidler-Mckay, PA., et al. 1996. Mol Cell Biol. 16: 4024-34. PMID: 8754800
9. The Gfi-1 proto-oncoprotein contains a novel transcriptional repressor domain, SNAG, and inhibits G1 arrest induced by interleukin-2 withdrawal.  |  Grimes, HL., et al. 1996. Mol Cell Biol. 16: 6263-72. PMID: 8887656
10. The Gfi-1 protooncoprotein represses Bax expression and inhibits T-cell death.  |  Grimes, HL., et al. 1996. Proc Natl Acad Sci U S A. 93: 14569-73. PMID: 8962093
11. The Gfi-1B proto-oncoprotein represses p21WAF1 and inhibits myeloid cell differentiation.  |  Tong, B., et al. 1998. Mol Cell Biol. 18: 2462-73. PMID: 9566867
12. The human homologue (GFI1B) of the chicken GFI gene maps to chromosome 9q34.13-A locus frequently altered in hematopoietic diseases.  |  Rödel, B., et al. 1998. Genomics. 54: 580-2. PMID: 9878267

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Gfi-1 Antibody (B-9) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Gfi-1 Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Gfi-1 Antibody (B-9) Specifications

Alternate Product Name: GFI1

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 41-240

Epitope Target: Gfi-1

Epitope Species: human

Product Reactivity/Detection: Gfi-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Gfi-1 Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent