GFAP Antibody (GA-5): sc-58766

GFAP Antibody (GA-5) is a mouse monoclonal IgG1 kappa light chain antibody engineered to target glial fibrillary acidic protein (GFAP), a pivotal intermediate filament protein integral to the structural integrity and function of astroglial cells in the central nervous system. GFAP, also known as ALXDRD, is characterized by its distinctive tripartite structure, which includes an amino-terminal head domain, a central α-helical rod domain essential for filament assembly, and a carboxy-terminal tail domain that mediates interactions with other cellular proteins and organelles. This structural configuration is critical for maintaining the resilience and adaptability of astrocytes, enabling them to respond effectively to neurological insults and maintain homeostasis within the brain and spinal cord. GFAP monoclonal antibody (GA-5) is raised against GFAP isolated from porcine spinal cord and exhibits broad species reactivity, making GFAP (GA-5) antibody suitable for detecting GFAP in mouse, rat, human, avian, bovine, porcine, feline, and rabbit tissues through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP) and frozen sections. GFAP (GA-5) monoclonal antibody is recommended for researchers studying astrogliosis, a process characterized by increased GFAP expression in response to conditions like mechanical trauma, AIDS related dementia, prion infections, and inflammatory demyelinating diseases, thereby providing essential insights into the pathophysiology of various central nervous system disorders. Anti-GFAP antibody (GA-5) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® variants.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GFAP Antibody (GA-5) has 35 citations in a variety of scientific publications.

Primary antibodies like Anti-GFAP Antibody (GA-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GFAP Antibody (GA-5) Specifications

Alternate Product Name: ALXDRD

Product Clone Name: GA-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: GFAP

Epitope Species: porcine

Product Reactivity/Detection: GFAP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine, porcine and feline, rabbit

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for GFAP Antibody (GA-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred GFAP antibody is GFAP Antibody (2E1): sc-33673.