Gephyrin Antibody (45) recommended for detection of Gephyrin of mouse, rat, human and Drosophila origin by WB, IP, IF and IHC(P)

Gephyrin Antibody (45): sc-135920

Gephyrin Antibody (45) is rated 4.7 out of 5 by 3.
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5
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Datasheets

    • Gephyrin Antibody (45) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 569-726 of Gephyrin of rat origin
    • recommended for detection of Gephyrin of mouse, rat, human and Drosophila origin by WB, IP, IF and IHC(P)

Can sc-135920: Gephyrin (45) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, sc-135920: Gephyrin (45) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Sensitive and specific Antibody used for Western blotting at 1:200. Results gave a single, clear band at the expected weight, even after reusing the antibody several times (storing the diluted Ab at -20C).
Date published: 2020-06-15
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using rat brain tissue extract. -SCBT QC
Date published: 2015-03-03
Rated 4 out of 5 by from Robust immunoperoxidase cytoplasmic staining Robust immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded rat cerebellum tissue. -SCBT QC
Date published: 2013-01-06
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