Gemin7 Antibody (H-9): sc-514950
- Gemin7 Antibody (H-9) is a mouse monoclonal IgG2a κ Gemin7 antibody provided at 200 µg/ml
- raised against amino acids 19-129 mapping at the C-terminus of Gemin7 of mouse origin
- Gemin7 Antibody (H-9) is recommended for detection of Gemin7 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-Gemin7 Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP is the preferred secondary detection reagent for Gemin7 Antibody (H-9) for WB applications. This reagent is now offered in a bundle with Gemin7 Antibody (H-9) (see ordering information below).
Gemin7 Antibody (H-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects Gemin7 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gemin7 (H-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gemin7 protein, consisting of 131 amino acids, is encoded by the mouse gene Gemin7 and plays a crucial role as a component of the large multiprotein survival of motor neuron (SMN) complex, which is essential for the assembly of small nuclear ribonucleoproteins (snRNPs) that are vital for pre-mRNA splicing. The SMN complex is particularly important in spinal muscular atrophy, a neurodegenerative disease, as SMN complex includes the survival of motor neurons (SMN) protein, which is directly linked to the pathology of this condition. Notably, Gemin7, along with Gemin6, adopts a canonical Sm fold despite lacking significant sequence similarity with Sm proteins, and forms a heterodimer that interacts through a similar interface to that of Sm proteins. This interaction is critical for the binding of the Gemin6/Gemin7 complex to Sm proteins, facilitating the organization of Sm proteins necessary for the formation of Sm rings on snRNA targets, thereby underscoring Gemin7′s importance in RNA processing and gene expression regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Gemin7 Antibody (H-9) References:
- Identification and characterization of Gemin7, a novel component of the survival of motor neuron complex. | Baccon, J., et al. 2002. J Biol Chem. 277: 31957-62. PMID: 12065586
- Gemin 6 and 7 lend a hand to snRNP assembly. | Leung, AK. and Nagai, K. 2005. Structure. 13: 833-4. PMID: 15939012
- The Gemin6-Gemin7 heterodimer from the survival of motor neurons complex has an Sm protein-like structure. | Ma, Y., et al. 2005. Structure. 13: 883-92. PMID: 15939020
- Gemin proteins are required for efficient assembly of Sm-class ribonucleoproteins. | Shpargel, KB. and Matera, AG. 2005. Proc Natl Acad Sci U S A. 102: 17372-7. PMID: 16301532
- Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons. | Zhang, H., et al. 2006. J Neurosci. 26: 8622-32. PMID: 16914688
- Synergistic inhibition of pancreatic adenocarcinoma cell growth by trichostatin A and gemcitabine. | Donadelli, M., et al. 2007. Biochim Biophys Acta. 1773: 1095-106. PMID: 17555830
- Ribonucleoprotein assembly defects correlate with spinal muscular atrophy severity and preferentially affect a subset of spliceosomal snRNPs. | Gabanella, F., et al. 2007. PLoS One. 2: e921. PMID: 17895963
- Analysis of the SIP3 protein identified in a two-hybrid screen for interaction with the SNF1 protein kinase. | Lesage, P., et al. 1994. Nucleic Acids Res. 22: 597-603. PMID: 8127709
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gemin7 Antibody (H-9) | sc-514950 | 200 µg/ml | $316.00 | |||
Gemin7 Antibody (H-9): m-IgG2a BP-HRP Bundle | sc-545980 | 200 µg Ab; 10 µg BP | $354.00 | |||
Gemin7 Antibody (H-9) AC | sc-514950 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Gemin7 Antibody (H-9) HRP | sc-514950 HRP | 200 µg/ml | $316.00 | |||
Gemin7 Antibody (H-9) FITC | sc-514950 FITC | 200 µg/ml | $330.00 | |||
Gemin7 Antibody (H-9) PE | sc-514950 PE | 200 µg/ml | $343.00 | |||
Gemin7 Antibody (H-9) Alexa Fluor® 488 | sc-514950 AF488 | 200 µg/ml | $357.00 | |||
Gemin7 Antibody (H-9) Alexa Fluor® 546 | sc-514950 AF546 | 200 µg/ml | $357.00 | |||
Gemin7 Antibody (H-9) Alexa Fluor® 594 | sc-514950 AF594 | 200 µg/ml | $357.00 | |||
Gemin7 Antibody (H-9) Alexa Fluor® 647 | sc-514950 AF647 | 200 µg/ml | $357.00 | |||
Gemin7 Antibody (H-9) Alexa Fluor® 680 | sc-514950 AF680 | 200 µg/ml | $357.00 | |||
Gemin7 Antibody (H-9) Alexa Fluor® 790 | sc-514950 AF790 | 200 µg/ml | $357.00 |