Gemin3 Antibody (12H12): sc-57007

Gemin3 Antibody (12H12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Gemin3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-Gemin3 antibody (12H12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gemin3, also known as DP103, DDX20, DEAD-box protein DP130, and DEAD/H box 20, is located on human chromosome 1p13.2 and plays a critical role in the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs) within the cytoplasm, which is essential for pre-mRNA splicing in the nucleus. Gemin3 is a key component of the SMN (survival of motor neuron) complex, which includes other proteins such as Gemin2, Gemin4, Gemin5, and Gemin6, and is vital for proper functioning of the spliceosome. Gemin3′s presence in both cytoplasm and nucleus, particularly in subnuclear bodies known as gems, underscores Gemin3′s importance in RNA metabolism and gene expression regulation. Gemin3 interacts with SmB, SmD2, and SmD3, and exhibits ATPase activity, suggesting a potential role as a RNA helicase. Gemin3 is expressed in various tissues, including B and T cell neuroblastoma-derived cell lines, malignant melanoma, and normal testis, while showing lower expression levels in colon, skeletal muscle, liver, kidney, and lung. Gemin3′s relevance in spinal muscular atrophy (SMA) highlights Gemin3′s significance in neurodegenerative diseases, making Gemin3 (12H12) monoclonal antibody an invaluable tool for researchers studying RNA processing and related pathologies.

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Gemin3 Antibody (12H12) References:

1. A single nucleotide difference that alters splicing patterns distinguishes the SMA gene SMN1 from the copy gene SMN2.  |  Monani, UR., et al. 1999. Hum Mol Genet. 8: 1177-83. PMID: 10369862
2. Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C.  |  Grundhoff, AT., et al. 1999. J Biol Chem. 274: 19136-44. PMID: 10383418
3. Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems.  |  Charroux, B., et al. 1999. J Cell Biol. 147: 1181-94. PMID: 10601333
4. Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins.  |  Meister, G., et al. 2000. Hum Mol Genet. 9: 1977-86. PMID: 10942426
5. SMN interacts with a novel family of hnRNP and spliceosomal proteins.  |  Mourelatos, Z., et al. 2001. EMBO J. 20: 5443-52. PMID: 11574476
6. SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro.  |  Todd, AG., et al. 2010. J Mol Biol. 401: 681-9. PMID: 20620147
7. NANOS1 and PUMILIO2 bind microRNA biogenesis factor GEMIN3, within chromatoid body in human germ cells.  |  Ginter-Matuszewska, B., et al. 2011. Histochem Cell Biol. 136: 279-87. PMID: 21800163
8. The multiple lives of DEAD-box RNA helicase DP103/DDX20/Gemin3.  |  Curmi, F. and Cauchi, RJ. 2018. Biochem Soc Trans. 46: 329-341. PMID: 29523774
9. SMN complex member Gemin3 self-interacts and has a functional relationship with ALS-linked proteins TDP-43, FUS and Sod1.  |  Cacciottolo, R., et al. 2019. Sci Rep. 9: 18666. PMID: 31822699
10. Survival motor neuron protein and neurite degeneration are regulated by Gemin3 in spinal muscular atrophy motoneurons.  |  Miralles, MP., et al. 2022. Front Cell Neurosci. 16: 1054270. PMID: 36619669
11. The survival motor neuron protein in spinal muscular atrophy.  |  Coovert, DD., et al. 1997. Hum Mol Genet. 6: 1205-14. PMID: 9259265
12. The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis.  |  Fischer, U., et al. 1997. Cell. 90: 1023-9. PMID: 9323130

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Gemin3 Antibody (12H12) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Gemin3 Antibody (12H12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Gemin3 Antibody (12H12) Specifications

Alternate Product Name: Gemin3

Product Clone Name: 12H12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 368-548

Epitope Target: Gemin3

Epitope Species: human

Product Reactivity/Detection: Gemin3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Gemin3 Antibody (12H12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin