Gemin2 Antibody (3F8): sc-32806

Gemin2 Antibody (3F8) is a mouse monoclonal IgG2b antibody that detects Gemin2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Gemin2 plays a crucial role in the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), which are essential for pre-mRNA splicing, a fundamental process in gene expression regulation. Gemin2 directly interacts with the survival of motor neuron (SMN) protein, forming a complex integral to snRNP biogenesis in the cytoplasm and subsequent transport to the nucleus. This interaction is particularly important in spinal muscular atrophy (SMA), where SMN gene mutations lead to SMN-Gemin2 complex deficiency, resulting in impaired snRNP assembly and motor neuron degeneration. Gemin2 is predominantly localized in both the cytoplasm and nucleus, with significant concentration in subnuclear structures known as gems, which are critical for snRNP storage and assembly. Additionally, Gemin2 expression can be induced by TGFβ treatment and is notably elevated in various E-cadherin negative human carcinoma cell lines, highlighting its potential role in cancer biology. Anti-Gemin2 antibody (3F8) is an invaluable tool for researchers studying the molecular mechanisms underlying SMA and the broader implications of splicing regulation in health and disease.

The preferred Gemin2 antibody is Gemin2 Antibody (1G9): sc-33703.