Gemin2 Antibody (1G9): sc-33703
- Gemin2 Antibody (1G9) is a mouse monoclonal IgG2b κ Gemin2 antibody provided at 200 µg/ml
- raised against recombinant human Gemin2
- Gemin2 Antibody (1G9) is recommended for detection of Gemin2 of mouse, rat and human origin by WB, IP and IF
- Anti-Gemin2 Antibody (1G9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Gemin2 Antibody (1G9) for WB applications. These reagents are now offered in bundles with Gemin2 Antibody (1G9) (see ordering information below).
Gemin2 Antibody (1G9) is a mouse monoclonal IgG2b kappa light chain antibody that detects Gemin2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Gemin2 (1G9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gemin2 plays a crucial role in the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), which are essential for pre-mRNA splicing, a fundamental process in gene expression regulation. Located in both the cytoplasm and nucleus, Gemin2 is particularly concentrated in subnuclear structures known as gems, which are involved in the storage and assembly of snRNPs. Gemin2′s interaction with the survival of motor neuron (SMN) complex, which includes proteins such as Gemin3, Gemin4, Gemin5, and Gemin6, is vital for proper functioning of the spliceosome. Disruption of this complex is implicated in spinal muscular atrophy (SMA), a severe neurodegenerative disorder characterized by the loss of motor neurons. Gemin2 is expressed in various tissues, with notable expression in the spinal cord, and Gemin2 levels can be influenced by factors such as TGFβ treatment, highlighting a potential role in cancer biology, particularly in E-cadherin negative human carcinoma cell lines.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Gemin2 Antibody (1G9) References:
- A single nucleotide difference that alters splicing patterns distinguishes the SMA gene SMN1 from the copy gene SMN2. | Monani, UR., et al. 1999. Hum Mol Genet. 8: 1177-83. PMID: 10369862
- Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. | Meister, G., et al. 2000. Hum Mol Genet. 9: 1977-86. PMID: 10942426
- The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion. | Comijn, J., et al. 2001. Mol Cell. 7: 1267-78. PMID: 11430829
- SMN interacts with a novel family of hnRNP and spliceosomal proteins. | Mourelatos, Z., et al. 2001. EMBO J. 20: 5443-52. PMID: 11574476
- The survival motor neuron protein in spinal muscular atrophy. | Coovert, DD., et al. 1997. Hum Mol Genet. 6: 1205-14. PMID: 9259265
- The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis. | Fischer, U., et al. 1997. Cell. 90: 1023-9. PMID: 9323130
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gemin2 Antibody (1G9) | sc-33703 | 200 µg/ml | $322.00 | |||
Gemin2 Antibody (1G9): m-IgG Fc BP-HRP Bundle | sc-528489 | 200 µg Ab; 10 µg BP | $361.00 | |||
Gemin2 Antibody (1G9): m-IgGκ BP-HRP Bundle | sc-520880 | 200 µg Ab, 40 µg BP | $361.00 | |||
Gemin2 Antibody (1G9): m-IgG2b BP-HRP Bundle | sc-550044 | 200 µg Ab; 10 µg BP | $361.00 | |||
Gemin2 Antibody (1G9) AC | sc-33703 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Gemin2 Antibody (1G9) HRP | sc-33703 HRP | 200 µg/ml | $322.00 | |||
Gemin2 Antibody (1G9) FITC | sc-33703 FITC | 200 µg/ml | $336.00 | |||
Gemin2 Antibody (1G9) PE | sc-33703 PE | 200 µg/ml | $349.00 | |||
Gemin2 Antibody (1G9) Alexa Fluor® 488 | sc-33703 AF488 | 200 µg/ml | $364.00 | |||
Gemin2 Antibody (1G9) Alexa Fluor® 546 | sc-33703 AF546 | 200 µg/ml | $364.00 | |||
Gemin2 Antibody (1G9) Alexa Fluor® 594 | sc-33703 AF594 | 200 µg/ml | $364.00 | |||
Gemin2 Antibody (1G9) Alexa Fluor® 647 | sc-33703 AF647 | 200 µg/ml | $364.00 | |||
Gemin2 Antibody (1G9) Alexa Fluor® 680 | sc-33703 AF680 | 200 µg/ml | $364.00 | |||
Gemin2 Antibody (1G9) Alexa Fluor® 790 | sc-33703 AF790 | 200 µg/ml | $364.00 |