Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Gelsolin CRISPR/Cas9 KO Plasmid (m): sc-432743
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gelsolin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Gelsolin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gelsolin Antibody (F-5): sc-514502
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gelsolin CRISPR/Cas9 KO Plasmid (m) | sc-432743 | 20 µg | $397.00 |
Overview
Mouse Gsn encodes gelsolin, a Ca2+-regulated actin-binding protein that severs, caps, and nucleates actin filaments to control cytoskeletal remodeling. By tuning actin dynamics, gelsolin contributes to cell shape changes, motility, endocytosis, and phagocytosis, and it interfaces with signaling networks such as phosphoinositide regulation and Rho family GTPase–linked pathways that couple membrane cues to actin reorganization. Altered gelsolin activity and expression have been associated with dysregulated cell migration, inflammatory responses, and tissue remodeling, making it a useful node for studying cytoskeleton-driven phenotypes. In mouse systems, Gsn perturbation supports mechanistic research on actin-dependent processes relevant to vascular biology, neurobiology, and immune cell function.
Gelsolin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gsn gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gsn together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gsn open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Gelsolin protein expression.
This CRISPR knockout system enables efficient generation of Gsn-deficient cell models for investigation of Gelsolin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gsn exon(s) critical for Gelsolin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gsn genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Gelsolin CRISPR/Cas9 KO Plasmid (m) and Gelsolin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gsn locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Gelsolin HDR Plasmid (m) and Gelsolin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gsn homology arms to support homology-directed repair at defined Gsn target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.