Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Gelsolin CRISPR/Cas9 KO Plasmid (h): sc-401005
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gelsolin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Gelsolin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gelsolin Antibody (F-5): sc-514502
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gelsolin CRISPR/Cas9 KO Plasmid (h) | sc-401005 | 20 µg | $397.00 |
Overview
GSN encodes gelsolin, a calcium-regulated actin-binding protein that severs and caps actin filaments to control cytoskeletal remodeling and cell mechanics. Through modulation of actin polymerization dynamics, gelsolin contributes to processes including cell motility, adhesion, endocytosis, and apoptosis, and it interfaces with signaling pathways that govern stress responses and membrane trafficking. Altered GSN expression or gelsolin activity has been associated with changes in tissue remodeling and inflammatory microenvironments, and is frequently studied in the context of cancer cell invasion, neurodegeneration, and cardiovascular biology. As a key regulator of actin filament turnover, gelsolin provides a tractable node for dissecting actin-dependent phenotypes across diverse human cell models.
Gelsolin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GSN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GSN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GSN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Gelsolin protein expression.
This CRISPR knockout system enables efficient generation of GSN-deficient cell models for investigation of Gelsolin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GSN exon(s) critical for Gelsolin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GSN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Gelsolin CRISPR/Cas9 KO Plasmid (h) and Gelsolin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GSN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Gelsolin HDR Plasmid (h) and Gelsolin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GSN homology arms to support homology-directed repair at defined GSN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.