GEFT CRISPR/Cas9 KO Plasmid (h): sc-406707
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GEFT CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GEFT genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GEFT CRISPR/Cas9 KO Plasmid (h) | sc-406707 | 20 µg | $397.00 |
Overview
ARHGEF25 encodes GEFT, a Rho guanine nucleotide exchange factor that activates Rho-family GTPases such as Rac1 and Cdc42 to coordinate actin cytoskeleton remodeling, cell polarity, and motility. GEFT-mediated signaling interfaces with Rho GTPase–dependent pathways controlling focal adhesion dynamics, membrane ruffling, and neurite outgrowth, linking extracellular cues to downstream transcriptional and morphological programs. Dysregulated Rho GTPase signaling and altered ARHGEF25 activity have been associated with aberrant migration and invasion phenotypes in cancer biology and with broader defects in cytoskeletal organization relevant to neurodevelopmental and inflammatory contexts. As a node upstream of multiple Rho-dependent effectors, GEFT is frequently interrogated to dissect mechanisms of morphogenesis, adhesion, and signal transduction.
GEFT CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ARHGEF25 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ARHGEF25 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ARHGEF25 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GEFT protein expression.
This CRISPR knockout system enables efficient generation of ARHGEF25-deficient cell models for investigation of GEFT signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ARHGEF25 exon(s) critical for GEFT function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ARHGEF25 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GEFT CRISPR/Cas9 KO Plasmid (h) and GEFT CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ARHGEF25 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GEFT HDR Plasmid (h) and GEFT HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ARHGEF25 homology arms to support homology-directed repair at defined ARHGEF25 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.