GDF-15 Antibody (H-2): sc-515675
- GDF-15 Antibody (H-2) is a mouse monoclonal IgG2a κ, cited in 12 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 41-62 near the N-terminus of GDF-15 of rat origin
- Anti-GDF-15 Antibody (H-2) is recommended for detection of precursor and mature GDF-15 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GDF-15 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GDF-15 Antibody (H-2) for WB applications. These reagents are now offered in bundles with GDF-15 Antibody (H-2) (see ordering information below).
GDF-15 Antibody (H-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects growth differentiation factor 15 (GDF-15) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GDF-15 antibody (H-2) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GDF-15, also known as PDF, MIC-1, PLAB, NAG-1, and PTGF-β, belongs to the transforming growth factor beta (TGF-beta) superfamily and plays a crucial role in cellular responses to stress and inflammation. GDF-15 is synthesized intracellularly and secreted as a dimer linked by disulfide bonds, with highest expression in epithelial cells and macrophages, while maintaining wide distribution in adult tissues. GDF-15 is secreted into the cerebrospinal fluid from the choroid plexus in the brain, highlighting importance in central nervous system function. p53 tumor suppressor protein regulates GDF-15 gene expression, and in cultured cerebellar granule neurons, GDF-15 prevents cell death by activating the Akt pathway while inhibiting ERK signaling. This trophic factor promotes cell survival and differentiation in specific neuronal populations. GDF-15 overexpression in prostate cancer may serve as a potential diagnostic marker. During pregnancy, GDF-15 may help suppress proinflammatory cytokines derived from the mother, enhancing fetal survival.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GDF-15 Antibody (H-2) References:
- The metabolic effects of GDF15 are mediated by the orphan receptor GFRAL. | Emmerson, PJ., et al. 2017. Nat Med. 23: 1215-1219. PMID: 28846098
- GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans. | Patel, S., et al. 2019. Cell Metab. 29: 707-718.e8. PMID: 30639358
- ARRB1 inhibits non-alcoholic steatohepatitis progression by promoting GDF15 maturation. | Zhang, Z., et al. 2020. J Hepatol. 72: 976-989. PMID: 31857195
- GDF15 mediates the metabolic effects of PPARβ/δ by activating AMPK. | Aguilar-Recarte, D., et al. 2021. Cell Rep. 36: 109501. PMID: 34380027
- A growth factor-expressing macrophage subpopulation orchestrates regenerative inflammation via GDF-15. | Patsalos, A., et al. 2022. J Exp Med. 219: PMID: 34846534
- Growth differentiation factor 15 (GDF15) and semaglutide inhibit food intake and body weight through largely distinct, additive mechanisms. | Ghidewon, M., et al. 2022. Diabetes Obes Metab. 24: 1010-1020. PMID: 35129264
- Growth differentiation factor 15 (GDF-15) in endocrinology. | Iglesias, P., et al. 2023. Endocrine. 81: 419-431. PMID: 37129758
- Hepatic glucuronyl C5-epimerase combats obesity by stabilising GDF15. | He, F., et al. 2023. J Hepatol. 79: 605-617. PMID: 37217020
- Growth differentiation factor 15 (GDF-15) in kidney diseases. | Delrue, C., et al. 2023. Adv Clin Chem. 114: 1-46. PMID: 37268330
- Macrophages as a Source and Target of GDF-15. | Silva-Bermudez, LS., et al. 2024. Int J Mol Sci. 25: PMID: 39000420
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GDF-15 Antibody (H-2) | sc-515675 | 200 µg/ml | $322.00 | |||
GDF-15 Antibody (H-2): m-IgG Fc BP-HRP Bundle | sc-531464 | 200 µg Ab; 10 µg BP | $361.00 | |||
GDF-15 Antibody (H-2): m-IgGκ BP-HRP Bundle | sc-525159 | 200 µg Ab, 40 µg BP | $361.00 | |||
GDF-15 Antibody (H-2): m-IgG2a BP-HRP Bundle | sc-547796 | 200 µg Ab; 10 µg BP | $361.00 | |||
GDF-15 Antibody (H-2) AC | sc-515675 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GDF-15 Antibody (H-2) HRP | sc-515675 HRP | 200 µg/ml | $322.00 | |||
GDF-15 Antibody (H-2) FITC | sc-515675 FITC | 200 µg/ml | $336.00 | |||
GDF-15 Antibody (H-2) PE | sc-515675 PE | 200 µg/ml | $349.00 | |||
GDF-15 Antibody (H-2) Alexa Fluor® 488 | sc-515675 AF488 | 200 µg/ml | $364.00 | |||
GDF-15 Antibody (H-2) Alexa Fluor® 546 | sc-515675 AF546 | 200 µg/ml | $364.00 | |||
GDF-15 Antibody (H-2) Alexa Fluor® 594 | sc-515675 AF594 | 200 µg/ml | $364.00 | |||
GDF-15 Antibody (H-2) Alexa Fluor® 647 | sc-515675 AF647 | 200 µg/ml | $364.00 | |||
GDF-15 Antibody (H-2) Alexa Fluor® 680 | sc-515675 AF680 | 200 µg/ml | $364.00 | |||
GDF-15 Antibody (H-2) Alexa Fluor® 790 | sc-515675 AF790 | 200 µg/ml | $364.00 |