GCSFR Double Nickase Plasmid (h): sc-402269-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- G-CSFR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- G-CSFR Double Nickase Plasmid (h) and G-CSFR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSF3R. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: G-CSFR Antibody (A-7): sc-514639
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCSFR Double Nickase Plasmid (h) | sc-402269-NIC | 20 µg | $410.00 | |||
G-CSFR Double Nickase Plasmid (h2) | sc-402269-NIC-2 | 20 µg | $410.00 |
CSF3R encodes the granulocyte colony-stimulating factor receptor (GCSFR), a type I cytokine receptor that regulates neutrophil production, maturation, and functional activation in the myeloid lineage. Upon ligand engagement, GCSFR signals primarily through JAK/STAT, PI3K/AKT, and MAPK cascades to control proliferation, survival, differentiation, and trafficking of granulocytic progenitors. Tight regulation of CSF3R signaling is essential for hematopoietic homeostasis, and altered receptor activity has been linked to disordered myelopoiesis and leukemic transformation in multiple disease contexts. Because CSF3R integrates cytokine cues with transcriptional programs controlling granulopoiesis, it is frequently studied in models of inflammation, innate immune development, and myeloid malignancy mechanisms.
G-CSFR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSF3R locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSF3R. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSF3R function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSF3R-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.