GCP4 Antibody (D-5): sc-271876

GCP4 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects GCP4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GCP4 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GCP4, also known as gamma-tubulin complex component 4, is a crucial 667 amino acid protein that plays a significant role in microtubule organization and nucleation, particularly during cell division. GCP4 is a key member of the γ-Tubulin complex, which includes other essential proteins such as GCP2, GCP3, GCP5, and GCP6, all of which are vital for the formation and function of microtubule organizing centers. GCP4 is predominantly localized to the metaphase spindle during mitosis, where GCP4 facilitates the proper alignment and separation of chromosomes. Notably, in response to proteasome inhibition, GCP4 accumulates at the pericentriolar material, enhancing GCP4′s role in microtubule dynamics. Precise regulation of GCP4 and interactions with other γ-Tubulin complex components are critical for maintaining cellular integrity and ensuring accurate cell division, making GCP4 an important target for research in cell biology and cancer studies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GCP4 Antibody (D-5) References:

1. Human 76p: A new member of the gamma-tubulin-associated protein family.  |  Fava, F., et al. 1999. J Cell Biol. 147: 857-68. PMID: 10562286
2. An unappreciated role for RNA surveillance.  |  Hillman, RT., et al. 2004. Genome Biol. 5: R8. PMID: 14759258
3. Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.  |  Rush, J., et al. 2005. Nat Biotechnol. 23: 94-101. PMID: 15592455
4. Microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function.  |  Delgehyr, N., et al. 2005. J Cell Sci. 118: 1565-75. PMID: 15784680
5. GCP-WD is a gamma-tubulin targeting factor required for centrosomal and chromatin-mediated microtubule nucleation.  |  Lüders, J., et al. 2006. Nat Cell Biol. 8: 137-47. PMID: 16378099
6. PhLP3 modulates CCT-mediated actin and tubulin folding via ternary complexes with substrates.  |  Stirling, PC., et al. 2006. J Biol Chem. 281: 7012-21. PMID: 16415341
7. Fluorescence resonance energy transfer and molecular modeling studies on 4',6-diamidino-2-phenylindole (DAPI) complexes with tubulin.  |  Arbildua, JJ., et al. 2006. Protein Sci. 15: 410-9. PMID: 16452620
8. Noncore components of the fission yeast gamma-tubulin complex.  |  Anders, A., et al. 2006. Mol Biol Cell. 17: 5075-93. PMID: 17021256
9. The mammalian gamma-tubulin complex contains homologues of the yeast spindle pole body components spc97p and spc98p.  |  Murphy, SM., et al. 1998. J Cell Biol. 141: 663-74. PMID: 9566967

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GCP4 Antibody (D-5) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-GCP4 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GCP4 Antibody (D-5) Specifications

Alternate Product Name: GCP4

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 11-310

Epitope Target: GCP4

Epitope Species: human

Product Reactivity/Detection: γ-Tubulin complex component 4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for GCP4 Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin