GCM1 Antibody (R-06): sc-101173

GCM1 Antibody (R-06) is a mouse monoclonal IgG2a kappa light chain antibody that detects GCM1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GCM1 antibody (R-06) is available as a non-conjugated format. GCM1, or glial cells missing homolog 1, is a 436 amino acid protein that serves as a crucial transcription factor in placental development. GCM1 is specifically localized to the nucleus and predominantly expressed in the placenta, where GCM1 plays a vital role in regulating gene expression necessary for trophoblast differentiation and placental formation. GCM1 binds to a unique DNA sequence (A/G)CCCGCAT, which is essential for transcriptional regulation of genes involved in placental development. GCM1 is implicated in regulating syncytin SU-mediated trophoblastic fusion, a critical process leading to syncytiotrophoblast formation, essential for proper placental functioning. A conserved N-terminal GCM DNA-binding domain within GCM1 enhances interaction with various transcription factors, influencing developmental processes, making GCM1 both a marker for placental tissues and a significant player in understanding placental biology and associated disorders.

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GCM1 Antibody (R-06) References:

1. A GCM motif protein is involved in placenta-specific expression of human aromatase gene.  |  Yamada, K., et al. 1999. J Biol Chem. 274: 32279-86. PMID: 10542267
2. Genomic organization, chromosomal localization, and the complete 22 kb DNA sequence of the human GCMa/GCM1, a placenta-specific transcription factor gene.  |  Yamada, K., et al. 2000. Biochem Biophys Res Commun. 278: 134-9. PMID: 11071865
3. GCMa regulates the syncytin-mediated trophoblastic fusion.  |  Yu, C., et al. 2002. J Biol Chem. 277: 50062-8. PMID: 12397062
4. Complex patterns of GCM1 mRNA and protein in villous and extravillous trophoblast cells of the human placenta.  |  Baczyk, D., et al. 2004. Placenta. 25: 553-9. PMID: 15135239
5. Stimulation of GCMa and syncytin via cAMP mediated PKA signaling in human trophoblastic cells under normoxic and hypoxic conditions.  |  Knerr, I., et al. 2005. FEBS Lett. 579: 3991-8. PMID: 16004993
6. Stimulation of GCMa transcriptional activity by cyclic AMP/protein kinase A signaling is attributed to CBP-mediated acetylation of GCMa.  |  Chang, CW., et al. 2005. Mol Cell Biol. 25: 8401-14. PMID: 16166624
7. Histone deacetylase 3 binds to and regulates the GCMa transcription factor.  |  Chuang, HC., et al. 2006. Nucleic Acids Res. 34: 1459-69. PMID: 16528103
8. Small ubiquitin-like modifier modification regulates the DNA binding activity of glial cell missing Drosophila homolog a.  |  Chou, CC., et al. 2007. J Biol Chem. 282: 27239-27249. PMID: 17646165
9. Gcm1 is involved in cell proliferation and fibrosis during kidney regeneration after ischemia-reperfusion injury.  |  Kamejima, S., et al. 2019. Sci Rep. 9: 7883. PMID: 31133638
10. Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation.  |  Wang, LJ., et al. 2022. Nat Commun. 13: 1626. PMID: 35338152
11. The multifaceted role of GCM1 during trophoblast differentiation in the human placenta.  |  Jeyarajah, MJ., et al. 2022. Proc Natl Acad Sci U S A. 119: e2203071119. PMID: 36442132
12. The gcm-motif: a novel DNA-binding motif conserved in Drosophila and mammals.  |  Akiyama, Y., et al. 1996. Proc Natl Acad Sci U S A. 93: 14912-6. PMID: 8962155

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GCM1 Antibody (R-06) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-GCM1 Antibody (R-06) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GCM1 Antibody (R-06) Specifications

Alternate Product Name: GCM1

Product Clone Name: R-06

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 108-166

Epitope Target: GCM1

Epitope Species: human

Product Reactivity/Detection: GCM1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for GCM1 Antibody (R-06): N/A