Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GCKR Double Nickase Plasmid (h): sc-401623-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GCKR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GCKR Double Nickase Plasmid (h) and GCKR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GCKR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GCKR Antibody (B-9): sc-166841
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCKR Double Nickase Plasmid (h) | sc-401623-NIC | 20 µg | $410.00 | |||
GCKR Double Nickase Plasmid (h2) | sc-401623-NIC-2 | 20 µg | $410.00 |
GCKR encodes glucokinase regulatory protein (GKRP), a hepatocyte-enriched modulator of glucose sensing that controls glucokinase (GCK) activity by sequestration and release in response to metabolic cues. Through regulation of hepatic glycolytic flux, glycogen synthesis, and de novo lipogenesis, GCKR influences central carbohydrate–lipid homeostasis and insulin-related signaling. Genetic variation or altered expression of GCKR has been associated with changes in fasting glucose, triglyceride levels, and broader metabolic trait phenotypes, making it a useful node for studying energy balance and nutrient-responsive transcriptional programs. Research on GCKR supports mechanistic interrogation of liver metabolism, endocrine crosstalk, and metabolic-stress responses in relevant cellular models.
GCKR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GCKR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GCKR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GCKR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GCKR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.