GC-C Double Nickase Plasmid (h): sc-403413-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GC-C Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GC-C Double Nickase Plasmid (h) and GC-C Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GUCY2C. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GC-C Antibody (537): sc-100302
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GC-C Double Nickase Plasmid (h) | sc-403413-NIC | 20 µg | $410.00 | |||
GC-C Double Nickase Plasmid (h2) | sc-403413-NIC-2 | 20 µg | $410.00 |
GUCY2C encodes guanylate cyclase C (GC-C), a transmembrane receptor that converts GTP to cGMP in response to extracellular ligands, thereby regulating epithelial ion transport and fluid homeostasis. GC-C-driven cGMP signaling engages downstream effectors such as PKG and cGMP-modulated phosphodiesterases to shape membrane transport, barrier function, and cell-state programs in mucosal tissues. Altered GUCY2C expression or signaling has been linked to dysregulated epithelial physiology and is studied in the context of intestinal inflammatory processes and epithelial transformation. As a lineage-associated epithelial receptor, GC-C is also used as a molecular handle to probe tissue-specific signaling responses and marker biology in human cell models.
GC-C Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GUCY2C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GUCY2C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GUCY2C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GUCY2C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.